Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture

Kód výsledku v IS VaVaI

RIV/00216275:25310/14:39896843 - isvavai.cz

Nalezeny alternativní kódy

RIV/61389013:_____/14:00427443

Výsledek na webu

http://dx.doi.org/10.1002/elps.201300196

DOI - Digital Object Identifier

10.1002/elps.201300196

Jazyk výsledku

angličtina

Název v původním jazyce

Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture

Popis výsledku v původním jazyce

In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells?specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied

Název v anglickém jazyce

Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture

Popis výsledku anglicky

In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells?specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

7E09109: Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

2-3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

7

Strana od-do

323-329

Kód UT WoS článku

000331899400012

EID výsledku v databázi Scopus

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