Application of immobilized trypsin reactors as proteomics tool. Techniques and procedures for disease diagnostics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39897997" target="_blank" >RIV/00216275:25310/14:39897997 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Application of immobilized trypsin reactors as proteomics tool. Techniques and procedures for disease diagnostics

Popis výsledku v původním jazyce

In proteomics study, trypsin is obviously the most often used immobilized proteinase for the preparation of peptide maps. We immobilized trypsin on cellulose nanofibers and on magnetic silica nanoparticles. Both enzyme reactors were applied for proteolytic digestion of model proteins (alfa-casein and bovine albumin). Analysis showed that immobilized trypsin is well usable and comfortable. There were good consensuses within protein recognition in comparison with soluble trypsin. Sequence coverage withinmagnetic silica nanoparticles functionalized by trypsin was higher than 47.6%. Sequence coverage for trypsin-nanofibers could not be determined due to high number of non-specific fragments.

Název v anglickém jazyce

Application of immobilized trypsin reactors as proteomics tool. Techniques and procedures for disease diagnostics

Popis výsledku anglicky

In proteomics study, trypsin is obviously the most often used immobilized proteinase for the preparation of peptide maps. We immobilized trypsin on cellulose nanofibers and on magnetic silica nanoparticles. Both enzyme reactors were applied for proteolytic digestion of model proteins (alfa-casein and bovine albumin). Analysis showed that immobilized trypsin is well usable and comfortable. There were good consensuses within protein recognition in comparison with soluble trypsin. Sequence coverage withinmagnetic silica nanoparticles functionalized by trypsin was higher than 47.6%. Sequence coverage for trypsin-nanofibers could not be determined due to high number of non-specific fragments.

Druh

C - Kapitola v odborné knize

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

—

Projekt

<a href="/cs/project/FR-TI1%2F436" target="_blank" >FR-TI1/436: *Využití nanovlákenných membrán pro řízené uvolňování aktivních látek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Techniques and procedures for disease diagnostics

ISBN

978-3-659-53330-3

Počet stran výsledku

8

Strana od-do

107-114

Počet stran knihy

124

Název nakladatele

LAP Lambert Academic Publishing AG & Co. KG

Místo vydání

Saarbrücken

Kód UT WoS kapitoly

—