Inorganic Silica Nanofibers in a Role of Bioactive Compound Carrier

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24220%2F15%3A00003403" target="_blank" >RIV/46747885:24220/15:00003403 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24620/15:00003403

Výsledek na webu

<a href="http://www.nanocon.eu/files/proceedings/23/papers/4485.pdf" target="_blank" >http://www.nanocon.eu/files/proceedings/23/papers/4485.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Inorganic Silica Nanofibers in a Role of Bioactive Compound Carrier

Popis výsledku v původním jazyce

This study is focused on enzyme immobilization on nanofibers (NF). NF are valued for their ultra-high specific surface areas and have been found potentially useful in many biomedical applications. A spectrum of bioactive molecules, including antibacterial agents, anti-cancer drugs, enzymes, proteins, can be incorporated into/onto nanofibers via different approaches. Enzyme immobilization providing many advantages over use of its soluble form: enhanced enzyme activity, reusability, simplified processing, better storage properties and pH stability. We describe the immobilization of trypsin (EC3.4.21.4) on silica nanofibers. After silanisation incorporating 72 ± 12.6 μg NH2 per mg of sheets, trypsin was bound using combinatory chemistry of EDAC and sulfo-NHS. The amount (215 ± 29.8 μg protein/cm2) and activity (24.8 ± 2.7 I.U./cm2) of immobilized biocatalyst as well as storage and operational stability were measured. The proteolytic activity of immobilized trypsin determined immediately after immobilization and during one week was high and stable. Prepared bio-functional nanofiber material can find the application in wound healing process (enzyme debridement).

Název v anglickém jazyce

Inorganic Silica Nanofibers in a Role of Bioactive Compound Carrier

Popis výsledku anglicky

This study is focused on enzyme immobilization on nanofibers (NF). NF are valued for their ultra-high specific surface areas and have been found potentially useful in many biomedical applications. A spectrum of bioactive molecules, including antibacterial agents, anti-cancer drugs, enzymes, proteins, can be incorporated into/onto nanofibers via different approaches. Enzyme immobilization providing many advantages over use of its soluble form: enhanced enzyme activity, reusability, simplified processing, better storage properties and pH stability. We describe the immobilization of trypsin (EC3.4.21.4) on silica nanofibers. After silanisation incorporating 72 ± 12.6 μg NH2 per mg of sheets, trypsin was bound using combinatory chemistry of EDAC and sulfo-NHS. The amount (215 ± 29.8 μg protein/cm2) and activity (24.8 ± 2.7 I.U./cm2) of immobilized biocatalyst as well as storage and operational stability were measured. The proteolytic activity of immobilized trypsin determined immediately after immobilization and during one week was high and stable. Prepared bio-functional nanofiber material can find the application in wound healing process (enzyme debridement).

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2015 - 7th International Conference on Nanomaterials - Research and Application

ISBN

978-80-87294-63-5

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

413-418

Název nakladatele

TANGER Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

1. 1. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000374708800073