Clostridial collagenase immobilized on chitosan nanofibers for burn healing
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39918517" target="_blank" >RIV/00216275:25310/22:39918517 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11150/22:10444659
Výsledek na webu
<a href="https://mmsl.cz/corproof.php?tartkey=mms-000000-0205" target="_blank" >https://mmsl.cz/corproof.php?tartkey=mms-000000-0205</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.31482/mmsl.2022.012" target="_blank" >10.31482/mmsl.2022.012</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Clostridial collagenase immobilized on chitosan nanofibers for burn healing
Popis výsledku v původním jazyce
This article describes the characterization and application of collagenase-based chitosan nanofiber membranes with rat burns. Electrospun chitosan nanofibers were functionalized with clostridial collagenase using carbodiimide chemistry. The immobilized collagenase was characterized by enzyme activity, kinetic constants, and dry storage stability measurements using a Pz-peptide substrate. The apparent kinetic constants KM and Vmax of immobilized collagenase showed a high affinity for the peptide substrate compared to the free enzyme. Drying of chitosan membranes with immobilized collagenase ensured 98% stability of enzyme activity after rehydration. The effect of collagenase immobilized on chitosan nanofibers on the burn of the rat model was compared with a control treatment with chitosan nanofibers. The healing of the wound with both materials was terminated after 30 days at the same time, although the collagenase wound healed more rapidly during healing. The scar area size after the application of collagenase-containing chitosan nanofiber membranes was 31.6% smaller than when only chitosan nanofibers were used.
Název v anglickém jazyce
Clostridial collagenase immobilized on chitosan nanofibers for burn healing
Popis výsledku anglicky
This article describes the characterization and application of collagenase-based chitosan nanofiber membranes with rat burns. Electrospun chitosan nanofibers were functionalized with clostridial collagenase using carbodiimide chemistry. The immobilized collagenase was characterized by enzyme activity, kinetic constants, and dry storage stability measurements using a Pz-peptide substrate. The apparent kinetic constants KM and Vmax of immobilized collagenase showed a high affinity for the peptide substrate compared to the free enzyme. Drying of chitosan membranes with immobilized collagenase ensured 98% stability of enzyme activity after rehydration. The effect of collagenase immobilized on chitosan nanofibers on the burn of the rat model was compared with a control treatment with chitosan nanofibers. The healing of the wound with both materials was terminated after 30 days at the same time, although the collagenase wound healed more rapidly during healing. The scar area size after the application of collagenase-containing chitosan nanofiber membranes was 31.6% smaller than when only chitosan nanofibers were used.
Klasifikace
Druh
J<sub>SC</sub> - Článek v periodiku v databázi SCOPUS
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Military Medical Science Letters
ISSN
0372-7025
e-ISSN
2571-113X
Svazek periodika
91
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
11
Strana od-do
324-334
Kód UT WoS článku
—
EID výsledku v databázi Scopus
2-s2.0-85143291488