Kinetic, stability, and activity of the nanoparticles-immobilized enzymes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F23%3A39919055" target="_blank" >RIV/00216275:25310/23:39919055 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/B978-0-323-95074-9.00001-4" target="_blank" >https://doi.org/10.1016/B978-0-323-95074-9.00001-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-323-95074-9.00001-4" target="_blank" >10.1016/B978-0-323-95074-9.00001-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Kinetic, stability, and activity of the nanoparticles-immobilized enzymes

Popis výsledku v původním jazyce

The article focuses on the catalytic activity and stability of enzymes immobilized on nanoparticles (NPs) for antimicrobial dressings and wound healing. The activity of immobilized enzymes is directly related to their performance, which is influenced by the immobilization process on solid supports. This literature review covers the bonding method as well as the different types and materials of NPs. The activity of immobilized enzymes was investigated in the field of enzyme kinetics, which is defined as the rate of enzyme catalysis. We compared the kinetic constants, the Michaelis constant ( K M), the maximum reaction rate ( V max), and the enzyme turnover number ( k cat), with native enzymes to see if the enzyme’s affinity to the substrate, and thus the steric availability of the enzyme binding site, did not change after immobilization.

Název v anglickém jazyce

Kinetic, stability, and activity of the nanoparticles-immobilized enzymes

Popis výsledku anglicky

The article focuses on the catalytic activity and stability of enzymes immobilized on nanoparticles (NPs) for antimicrobial dressings and wound healing. The activity of immobilized enzymes is directly related to their performance, which is influenced by the immobilization process on solid supports. This literature review covers the bonding method as well as the different types and materials of NPs. The activity of immobilized enzymes was investigated in the field of enzyme kinetics, which is defined as the rate of enzyme catalysis. We compared the kinetic constants, the Michaelis constant ( K M), the maximum reaction rate ( V max), and the enzyme turnover number ( k cat), with native enzymes to see if the enzyme’s affinity to the substrate, and thus the steric availability of the enzyme binding site, did not change after immobilization.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Posilování mezioborové spolupráce ve výzkumu nanomateriálů a při studiu jejich účinků na živé organismy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Antimicrobial dressings : the wound care applications

ISBN

978-0-323-95074-9

Počet stran výsledku

20

Strana od-do

133-152

Počet stran knihy

270

Název nakladatele

Elsevier Inc.

Místo vydání

San Diego

Kód UT WoS kapitoly

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