Modeling of lipase-catalyzed oil hydrolysis in supercritical CO2 in a packed-bed reactor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F22%3A00558422" target="_blank" >RIV/67985858:_____/22:00558422 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0332101" target="_blank" >http://hdl.handle.net/11104/0332101</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.supflu.2022.105629" target="_blank" >10.1016/j.supflu.2022.105629</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Modeling of lipase-catalyzed oil hydrolysis in supercritical CO2 in a packed-bed reactor

Popis výsledku v původním jazyce

Research on enzyme-catalyzed reactions of oils in supercritical CO2 (scCO(2)) aims at solvent-free natural products enriched in bioactive substances. Easy tailoring of scCO(2) properties and low mass transfer limitations belong to advantages of the method, which perspective of large-scale application depends on enzyme stability in scCO(2). Mathematical model for immobilized lipase-catalyzed vegetable oil hydrolysis in a continuous-flow reactor involves a slow reversible decrease of enzyme activity caused by glycerol retention in the enzyme bed. Reaction kinetics is modeled as pseudo-first-order and pseudo-second-order reversible hydrolysis of tri-, di-, and monoglycerides. Reaction rate constants and parameters of glycerol adsorption were estimated by fitting the model to previously published experimental data. Conditions of hydrolysis catalyzed by Lipozyme TL IM were pressure 10-30 MPa, temperature 30-50 degrees C, and scCO(2) flow rate-to-enzyme load ratio 2-120 g.g(-1) min(-1). The rate controlling step was hydrolysis of triglycerides with pressure and temperature dependent kinetic constant 100-230 min(-1).

Název v anglickém jazyce

Modeling of lipase-catalyzed oil hydrolysis in supercritical CO2 in a packed-bed reactor

Popis výsledku anglicky

Research on enzyme-catalyzed reactions of oils in supercritical CO2 (scCO(2)) aims at solvent-free natural products enriched in bioactive substances. Easy tailoring of scCO(2) properties and low mass transfer limitations belong to advantages of the method, which perspective of large-scale application depends on enzyme stability in scCO(2). Mathematical model for immobilized lipase-catalyzed vegetable oil hydrolysis in a continuous-flow reactor involves a slow reversible decrease of enzyme activity caused by glycerol retention in the enzyme bed. Reaction kinetics is modeled as pseudo-first-order and pseudo-second-order reversible hydrolysis of tri-, di-, and monoglycerides. Reaction rate constants and parameters of glycerol adsorption were estimated by fitting the model to previously published experimental data. Conditions of hydrolysis catalyzed by Lipozyme TL IM were pressure 10-30 MPa, temperature 30-50 degrees C, and scCO(2) flow rate-to-enzyme load ratio 2-120 g.g(-1) min(-1). The rate controlling step was hydrolysis of triglycerides with pressure and temperature dependent kinetic constant 100-230 min(-1).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

<a href="/cs/project/GA19-19245S" target="_blank" >GA19-19245S: Studie biokatalýzy v superkritickém oxidu uhličitém: obohacení rostlinných olejů esenciálními mastnými kyselinami</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Supercritical Fluids

ISSN

0896-8446

e-ISSN

1872-8162

Svazek periodika

187

Číslo periodika v rámci svazku

AUG 22

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

105629

Kód UT WoS článku

000804478400002

EID výsledku v databázi Scopus

2-s2.0-85130402836