TESTING OF COLLAGENASE COVALENTLY LINKED TO CHITOSAN NANOFIBERS FOR BIOMEDICAL APPLICATIONS

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

TESTING OF COLLAGENASE COVALENTLY LINKED TO CHITOSAN NANOFIBERS FOR BIOMEDICAL APPLICATIONS

Popis výsledku v původním jazyce

Nanofibers biofunctionalized by enzymes is materials of interest in the biomedical field due to their potential application in wound healing. This work describes the production and characterisation of electrospun chitosan nanofibers biofunctionalized bymicrobial collagenase. The morphology and microstructure of the chitosan nanofibers prepared by Nanospider technology were examined using a scanning electron microscopy (SEM). Amino groups of chitosan nanofiber were connected with the carboxylic acid groups of collagenase using EDAC and sulfo-NHS methodology. Collagenase enzymatic activity was evaluated by peptidic substrate (Pz-peptide, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg) in order to estimate the ability of this biomaterial to be used as an enzymatic debriding wound dressing. Characterisation of collagenase chitosan nanofibers in term of apparent Michaelis-Menten constants (KM(app) and vmax) measurements revealed higher enzyme to substrate affinity in comparison with so

Název v anglickém jazyce

TESTING OF COLLAGENASE COVALENTLY LINKED TO CHITOSAN NANOFIBERS FOR BIOMEDICAL APPLICATIONS

Popis výsledku anglicky

Nanofibers biofunctionalized by enzymes is materials of interest in the biomedical field due to their potential application in wound healing. This work describes the production and characterisation of electrospun chitosan nanofibers biofunctionalized bymicrobial collagenase. The morphology and microstructure of the chitosan nanofibers prepared by Nanospider technology were examined using a scanning electron microscopy (SEM). Amino groups of chitosan nanofiber were connected with the carboxylic acid groups of collagenase using EDAC and sulfo-NHS methodology. Collagenase enzymatic activity was evaluated by peptidic substrate (Pz-peptide, 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg) in order to estimate the ability of this biomaterial to be used as an enzymatic debriding wound dressing. Characterisation of collagenase chitosan nanofibers in term of apparent Michaelis-Menten constants (KM(app) and vmax) measurements revealed higher enzyme to substrate affinity in comparison with so

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Nanocon 2015: Conference Proceedings

ISBN

9788087294635

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

1-6

Název nakladatele

TANGER, spol. s r.o.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

14. 10. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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