Immobilization of enzymes on the silica nanofibers for burns treatment

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/46747885:24530/16:00004059

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immobilization of enzymes on the silica nanofibers for burns treatment

Popis výsledku v původním jazyce

For burns and necrotic wounds, removal of the necrotic tissue is the first step to successful treatment. Effective wound cleansing and debridement are essential for granulation and re-epithalisation. Now it is possible to replace a current very painful surgical removal with the enzymatic or autolytic debridement. Especially enzymatic debridement is a highly selective method that utilizes naturally occurring proteolytic enzymes. The reasons why the surgical removal has not yet been replaced by painless enzymatic debridement are low enzyme stability and many factors affecting enzymes activity (type of substrate, pH, temperature, etc.). The possibility to solve this problem is the enzyme immobilization on the suitable material - e.g. nanofibers with a lot of functional groups for covalent binding of the enzymes. As a supporting biocompatible and biodegradable material for immobilization of several enzymes types, the electrospun silica nanofibers were chosen. Proteolytic activity of immobilized enzymes was tested under different conditions.

Název v anglickém jazyce

Immobilization of enzymes on the silica nanofibers for burns treatment

Popis výsledku anglicky

For burns and necrotic wounds, removal of the necrotic tissue is the first step to successful treatment. Effective wound cleansing and debridement are essential for granulation and re-epithalisation. Now it is possible to replace a current very painful surgical removal with the enzymatic or autolytic debridement. Especially enzymatic debridement is a highly selective method that utilizes naturally occurring proteolytic enzymes. The reasons why the surgical removal has not yet been replaced by painless enzymatic debridement are low enzyme stability and many factors affecting enzymes activity (type of substrate, pH, temperature, etc.). The possibility to solve this problem is the enzyme immobilization on the suitable material - e.g. nanofibers with a lot of functional groups for covalent binding of the enzymes. As a supporting biocompatible and biodegradable material for immobilization of several enzymes types, the electrospun silica nanofibers were chosen. Proteolytic activity of immobilized enzymes was tested under different conditions.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2016 - Conference Proceedings, 8th International Conference on Nanomaterials - Research and Application

ISBN

9788087294710

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

509-513

Název nakladatele

TANGER Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

1. 1. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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