Immobilization of Proteolytic Enzymes onto Silica Nanofibers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24510%2F18%3A00007503" target="_blank" >RIV/46747885:24510/18:00007503 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24530/18:00007503

Výsledek na webu

<a href="http://vat.ft.tul.cz/Archive/VaT_2018_4.html" target="_blank" >http://vat.ft.tul.cz/Archive/VaT_2018_4.html</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immobilization of Proteolytic Enzymes onto Silica Nanofibers

Popis výsledku v původním jazyce

Even in modern medicine, it is still necessary to remove necrotic tissue from burns in a very painful method – by using surgical instruments. It is possible to replace the surgical method with application of proteolytic enzymes directly to the wound, which removes the necrotic tissue completely painlessly. However, most enzymes are active only for a short time and under the specific conditions. The catalytic activity of enzymes can be increased e.g. by immobilization of enzymes onto the biocompatible silica nanofibers. The nanofibers must be functionalized by suitable reagents to form a ligand between silica nanofibers and amino groups of proteolytic enzymes. In our research, the nanofibers surface was modified by 3-Aminopropyl triethoxysilane firstly, than functionalization by succinic anhydride and N-Hydroxysuccinimide ester was done. As the next step, 7 different proteolytic enzymes were immobilized to the silica nanofibers surface. Proteolytic activity of enzymes was tested under conditions simulated skin burns environment (temperature of 37°C and pH 4.6).

Název v anglickém jazyce

Immobilization of Proteolytic Enzymes onto Silica Nanofibers

Popis výsledku anglicky

Even in modern medicine, it is still necessary to remove necrotic tissue from burns in a very painful method – by using surgical instruments. It is possible to replace the surgical method with application of proteolytic enzymes directly to the wound, which removes the necrotic tissue completely painlessly. However, most enzymes are active only for a short time and under the specific conditions. The catalytic activity of enzymes can be increased e.g. by immobilization of enzymes onto the biocompatible silica nanofibers. The nanofibers must be functionalized by suitable reagents to form a ligand between silica nanofibers and amino groups of proteolytic enzymes. In our research, the nanofibers surface was modified by 3-Aminopropyl triethoxysilane firstly, than functionalization by succinic anhydride and N-Hydroxysuccinimide ester was done. As the next step, 7 different proteolytic enzymes were immobilized to the silica nanofibers surface. Proteolytic activity of enzymes was tested under conditions simulated skin burns environment (temperature of 37°C and pH 4.6).

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fibres and textiles (Vlákna a textil)

ISSN

1335-0617

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

4

Strana od-do

16-19

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-85064514920