Silica Nanofibers with Immobilized Proteolytic Enzyme for Dermal Applications

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/46747885:24530/19:00006519

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Silica Nanofibers with Immobilized Proteolytic Enzyme for Dermal Applications

Popis výsledku v původním jazyce

The necrotic tissue emerging in certain types of wounds is necessary to remove in the first wound healing phase. Removing of this tissue is called the debridement. Very painful surgical debridement can be replaced by painless enzyme debridement if we solve the problem with very low enzyme stability. For this reason, the proteolytic enzymes were immobilized on the surface of silica nanofibers. Silica nanofibers were produced by electrospinning from free liquid surface and then modify by silanization reaction. Three silanes were tested for silanization (3-Aminopropyl triethoxysilane, 3-Glycidyloxypropyl trimethoxysilane and 3-Mercaptopropyl trimethoxysilane). A two-step method with succinic anhydride and N-Hydroxysuccinimide ester was used for functionalization of the silica nanofibers. After this procedure, protease from Bacillus licheniformis, trypsin from hog pankreas and bromelain from pineapple stem were immobilized on functionalized silica nanofibers. The proteolytic activity of immobilized enzymes was tested in dependence on pH value and temperature. The most dependent on pH was bromelain from pineapple stem and the most dependent on temperature was protease from Bacillus licheniformis. As a comparative method for functionalizing the surface of nanofibres, a well-known method with glutaraldehyde was used. The proteolytic activity of immobilized enzymes with succinic anhydride and N-hydroxysuccinimide ester as a ligands was comparable or higher than the proteolytic activity of immobilized enzymes using glutaraldehyde as a ligand.

Název v anglickém jazyce

Silica Nanofibers with Immobilized Proteolytic Enzyme for Dermal Applications

Popis výsledku anglicky

The necrotic tissue emerging in certain types of wounds is necessary to remove in the first wound healing phase. Removing of this tissue is called the debridement. Very painful surgical debridement can be replaced by painless enzyme debridement if we solve the problem with very low enzyme stability. For this reason, the proteolytic enzymes were immobilized on the surface of silica nanofibers. Silica nanofibers were produced by electrospinning from free liquid surface and then modify by silanization reaction. Three silanes were tested for silanization (3-Aminopropyl triethoxysilane, 3-Glycidyloxypropyl trimethoxysilane and 3-Mercaptopropyl trimethoxysilane). A two-step method with succinic anhydride and N-Hydroxysuccinimide ester was used for functionalization of the silica nanofibers. After this procedure, protease from Bacillus licheniformis, trypsin from hog pankreas and bromelain from pineapple stem were immobilized on functionalized silica nanofibers. The proteolytic activity of immobilized enzymes was tested in dependence on pH value and temperature. The most dependent on pH was bromelain from pineapple stem and the most dependent on temperature was protease from Bacillus licheniformis. As a comparative method for functionalizing the surface of nanofibres, a well-known method with glutaraldehyde was used. The proteolytic activity of immobilized enzymes with succinic anhydride and N-hydroxysuccinimide ester as a ligands was comparable or higher than the proteolytic activity of immobilized enzymes using glutaraldehyde as a ligand.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů