Comparison of Mass Spectrometry Based Approaches in Lipidomics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899340" target="_blank" >RIV/00216275:25310/14:39899340 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of Mass Spectrometry Based Approaches in Lipidomics

Popis výsledku v původním jazyce

Lipidomics deals with the complex lipidomic characterization of dynamic biological systems. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but on the other hand the advantage is that the sample is infused at the constant eluent composition and flow rate, so the suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is another techniques, which is often used without a chromatographic separation, but the well-known limitation of MALDI for the quantitative purposes is less signal stability and reproducibility depending on the matrix used and the sample preparation. The systematic optimization of matrix selection and deposition approaches (spraying, electrospraying and sublimation) or matrix-free techniques on dedicated metal plates shows a potential of MALDI/LDI for the (semi-)quantitativ

Název v anglickém jazyce

Comparison of Mass Spectrometry Based Approaches in Lipidomics

Popis výsledku anglicky

Lipidomics deals with the complex lipidomic characterization of dynamic biological systems. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but on the other hand the advantage is that the sample is infused at the constant eluent composition and flow rate, so the suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is another techniques, which is often used without a chromatographic separation, but the well-known limitation of MALDI for the quantitative purposes is less signal stability and reproducibility depending on the matrix used and the sample preparation. The systematic optimization of matrix selection and deposition approaches (spraying, electrospraying and sublimation) or matrix-free techniques on dedicated metal plates shows a potential of MALDI/LDI for the (semi-)quantitativ

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/LL1302" target="_blank" >LL1302: Hmotnostní spektrometrie při hledání lipidových biomarkerů pro včasnou diagnostiku rakoviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů