Continuous comprehensive two-dimensional liquid chromatography-electrospray ionization mass spectrometry of complex lipidomic samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39899694" target="_blank" >RIV/00216275:25310/15:39899694 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00216-015-8528-2" target="_blank" >http://dx.doi.org/10.1007/s00216-015-8528-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-015-8528-2" target="_blank" >10.1007/s00216-015-8528-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Continuous comprehensive two-dimensional liquid chromatography-electrospray ionization mass spectrometry of complex lipidomic samples

Popis výsledku v původním jazyce

A new continuous comprehensive two-dimensional liquid chromatography-electrospray ionization mass spectrometry method has been developed for the lipidomic characterization of complex biological samples. The reversed-phase ultra-high-performance liquid chromatography with a C-18 column (150 mm x 1 mm, 1.7 mu m) used in the first dimension makes the separation of numerous lipid species differing in their hydrophobic part of the molecule, mainly fatty acyl chain lengths and the number and positions of double bonds, possible. Coeluted lipid species in the first dimension are resolved by the fast hydrophilic interaction liquid chromatography separation (50 mm x 3 mm, 2.7 mu m, core-shell particles) of lipid classes according to their different polarities inthe second dimension. Retention times in both dimensions, accurate m/z values, and tandem mass spectra provide high confidence in the identification of lipid species. The retention behavior of individual lipids in reversed-phase mode fol

Název v anglickém jazyce

Continuous comprehensive two-dimensional liquid chromatography-electrospray ionization mass spectrometry of complex lipidomic samples

Popis výsledku anglicky

A new continuous comprehensive two-dimensional liquid chromatography-electrospray ionization mass spectrometry method has been developed for the lipidomic characterization of complex biological samples. The reversed-phase ultra-high-performance liquid chromatography with a C-18 column (150 mm x 1 mm, 1.7 mu m) used in the first dimension makes the separation of numerous lipid species differing in their hydrophobic part of the molecule, mainly fatty acyl chain lengths and the number and positions of double bonds, possible. Coeluted lipid species in the first dimension are resolved by the fast hydrophilic interaction liquid chromatography separation (50 mm x 3 mm, 2.7 mu m, core-shell particles) of lipid classes according to their different polarities inthe second dimension. Retention times in both dimensions, accurate m/z values, and tandem mass spectra provide high confidence in the identification of lipid species. The retention behavior of individual lipids in reversed-phase mode fol

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

407

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

5033-5043

Kód UT WoS článku

000356461800014

EID výsledku v databázi Scopus

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