Influence of enzymes desaturases and elongases on lipid metabolism by type 2 diabetes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900000" target="_blank" >RIV/00216275:25310/15:39900000 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of enzymes desaturases and elongases on lipid metabolism by type 2 diabetes

Popis výsledku v původním jazyce

Objectives: This study evaluates the activity of D5, D6, D9 desaturases and elongases in all classes of lipids in diabetic patients and control group. We processed dependency of individual monitored enzymes on the content of glycated hemoglobin. Finally,the study includes statistical evaluation of measured values of fatty acids and available biochemical markers. Methods: Lipid extracts from deproteinated plasma samples were separated by preparative thin-layer chromatography into five fractions (phospholipids, diacylglycerols, free fatty acids, triglycerides and cholesterol esters). All fractions were isolated and after the addition of internal standard (cis-13,16,19-docosatrienoic acid, 10 ?g / ml) hydrolyzed and converted to corresponding methyl esters of fatty acids. Thus prepared samples were analyzed using a gas chromatograph. Conclusions: From the results obtained it is evident that the activity D9 desaturase is similar in diabetic and control groups. They also found a correlatio

Název v anglickém jazyce

Influence of enzymes desaturases and elongases on lipid metabolism by type 2 diabetes

Popis výsledku anglicky

Objectives: This study evaluates the activity of D5, D6, D9 desaturases and elongases in all classes of lipids in diabetic patients and control group. We processed dependency of individual monitored enzymes on the content of glycated hemoglobin. Finally,the study includes statistical evaluation of measured values of fatty acids and available biochemical markers. Methods: Lipid extracts from deproteinated plasma samples were separated by preparative thin-layer chromatography into five fractions (phospholipids, diacylglycerols, free fatty acids, triglycerides and cholesterol esters). All fractions were isolated and after the addition of internal standard (cis-13,16,19-docosatrienoic acid, 10 ?g / ml) hydrolyzed and converted to corresponding methyl esters of fatty acids. Thus prepared samples were analyzed using a gas chromatograph. Conclusions: From the results obtained it is evident that the activity D9 desaturase is similar in diabetic and control groups. They also found a correlatio

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

4th International Miniconference of PhD Students: When Biochemistry Meets Pharmacology and Toxicology

ISBN

978-80-971042-5-2

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

37-41

Název nakladatele

Ústav experimentálnej farmakológie a toxikológie SAV

Místo vydání

Bratislava

Místo konání akce

Bratislava

Datum konání akce

24. 6. 2015

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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