Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900025" target="_blank" >RIV/00216275:25310/15:39900025 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.4149/BLL_2015_004" target="_blank" >http://dx.doi.org/10.4149/BLL_2015_004</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4149/BLL_2015_004" target="_blank" >10.4149/BLL_2015_004</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers
Popis výsledku v původním jazyce
Objectives: The aim of this study was to establish and validate a high-performance liquid chromatography method for the determination of malondialdehyde in seminal plasma in smokers and non-smokers and to find possible differences between the two groups.Background: Malondialdehyde is used as a diagnostic marker of lipid peroxidation and indicator of oxidative stress. Smoking is suspected to be responsible for an increase in its level. Malondialdehyde has been thought to have cytotoxic and damaging effects. Methods: Semen samples were obtained from male partners of couples requesting a fertility evaluation. Malondialdehyde was derivatized with 2-thiobarbituric acid. The malondialdehyde-2-thiobarbituric acid complex was determined by liquid chromatography with fluorescence detection. The mobile phase consisted of 20% ethanol in 25-mmol/L potassium dihydrogenphosphate (v/v), pH 6.00 +- 0.05. Results: Analytical performance was satisfactory. Malondialdehyde levels were as follows: 1.50 +-
Název v anglickém jazyce
Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers
Popis výsledku anglicky
Objectives: The aim of this study was to establish and validate a high-performance liquid chromatography method for the determination of malondialdehyde in seminal plasma in smokers and non-smokers and to find possible differences between the two groups.Background: Malondialdehyde is used as a diagnostic marker of lipid peroxidation and indicator of oxidative stress. Smoking is suspected to be responsible for an increase in its level. Malondialdehyde has been thought to have cytotoxic and damaging effects. Methods: Semen samples were obtained from male partners of couples requesting a fertility evaluation. Malondialdehyde was derivatized with 2-thiobarbituric acid. The malondialdehyde-2-thiobarbituric acid complex was determined by liquid chromatography with fluorescence detection. The mobile phase consisted of 20% ethanol in 25-mmol/L potassium dihydrogenphosphate (v/v), pH 6.00 +- 0.05. Results: Analytical performance was satisfactory. Malondialdehyde levels were as follows: 1.50 +-
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Bratislava Medical Journal - Bratislavske Lekarske Listy
ISSN
0006-9248
e-ISSN
—
Svazek periodika
116
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
SK - Slovenská republika
Počet stran výsledku
5
Strana od-do
20-24
Kód UT WoS článku
000349275900004
EID výsledku v databázi Scopus
—