Optimized rinsing procedures for enhancing removal of residues of highly viscous and colored substances in the Bovine Corneal Opacity and Permeability (BCOP) assay
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900144" target="_blank" >RIV/00216275:25310/15:39900144 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.tiv.2015.05.019" target="_blank" >http://dx.doi.org/10.1016/j.tiv.2015.05.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tiv.2015.05.019" target="_blank" >10.1016/j.tiv.2015.05.019</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Optimized rinsing procedures for enhancing removal of residues of highly viscous and colored substances in the Bovine Corneal Opacity and Permeability (BCOP) assay
Popis výsledku v původním jazyce
Two modifications of the rinsing procedure within the BCOP assay were proposed. Their ability to enhance the removal efficiency of highly viscous and colored samples was compared with an unmodified BCOP procedure (TG OECD 437). The first modification consisted of three-step washing of the applied chemicals from the cornea using Eagle's Minimum Essential Medium (EMEM), olive oil and EMEM, while the classical OECD TG 437 procedure prescribes only EMEM. Within the second modification, mechanical removal ofthe tested sample from the cornea surface prior to the two step washing procedure was performed. The in vitro irritation score (IVIS) exceeded the value of 55 for 9 out of 20 samples when a non-modified rinsing procedure was used. The first modificationwith the olive oil resulted in a decrease in MS for numerous samples, while an IVIS score drop below the threshold value of 55 was only observed for two of them. Mechanical removal of sample residua resulted in a further decline in the m
Název v anglickém jazyce
Optimized rinsing procedures for enhancing removal of residues of highly viscous and colored substances in the Bovine Corneal Opacity and Permeability (BCOP) assay
Popis výsledku anglicky
Two modifications of the rinsing procedure within the BCOP assay were proposed. Their ability to enhance the removal efficiency of highly viscous and colored samples was compared with an unmodified BCOP procedure (TG OECD 437). The first modification consisted of three-step washing of the applied chemicals from the cornea using Eagle's Minimum Essential Medium (EMEM), olive oil and EMEM, while the classical OECD TG 437 procedure prescribes only EMEM. Within the second modification, mechanical removal ofthe tested sample from the cornea surface prior to the two step washing procedure was performed. The in vitro irritation score (IVIS) exceeded the value of 55 for 9 out of 20 samples when a non-modified rinsing procedure was used. The first modificationwith the olive oil resulted in a decrease in MS for numerous samples, while an IVIS score drop below the threshold value of 55 was only observed for two of them. Mechanical removal of sample residua resulted in a further decline in the m
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
DN - Vliv životního prostředí na zdraví
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Toxicology in Vitro
ISSN
0887-2333
e-ISSN
—
Svazek periodika
29
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
7
Strana od-do
1385 - 1391
Kód UT WoS článku
000361263500011
EID výsledku v databázi Scopus
—