Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900593" target="_blank" >RIV/00216275:25310/15:39900593 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023752:_____/15:43914801 RIV/00216208:11120/15:43910767

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jneuroim.2015.10.017" target="_blank" >http://dx.doi.org/10.1016/j.jneuroim.2015.10.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jneuroim.2015.10.017" target="_blank" >10.1016/j.jneuroim.2015.10.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product

Popis výsledku v původním jazyce

The latest therapeutic approaches to Alzheimer disease are using intravenous immunoglobulin (MG) products. Therefore, the detailed characterization of target-specific antibodies naturally occurring in MG products is beneficial. We have focused on characterization of antibodies isolated against tau protein, a biomarker of Alzheimer's disease, from Flebogamma IVIG product. The analysis of IgG subclass distribution indicated skewing toward IgG3 in anti-tau-enriched IgG fraction. The evaluation of their reactivity and avidity with several recombinant tau forms was performed by ELISA and blotting techniques. Truncated non-phosphotylated tau protein (amino acids 155-421) demonstrated the highest reactivity and avidity index. We provide the first detailed insight into the reactivity of isolated natural antibodies against tau protein.

Název v anglickém jazyce

Identification and characterization of natural antibodies against tau protein in an intravenous immunoglobulin product

Popis výsledku anglicky

The latest therapeutic approaches to Alzheimer disease are using intravenous immunoglobulin (MG) products. Therefore, the detailed characterization of target-specific antibodies naturally occurring in MG products is beneficial. We have focused on characterization of antibodies isolated against tau protein, a biomarker of Alzheimer's disease, from Flebogamma IVIG product. The analysis of IgG subclass distribution indicated skewing toward IgG3 in anti-tau-enriched IgG fraction. The evaluation of their reactivity and avidity with several recombinant tau forms was performed by ELISA and blotting techniques. Truncated non-phosphotylated tau protein (amino acids 155-421) demonstrated the highest reactivity and avidity index. We provide the first detailed insight into the reactivity of isolated natural antibodies against tau protein.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Neuroimmunology

ISSN

0165-5728

e-ISSN

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Svazek periodika

289

Číslo periodika v rámci svazku

October 28, 2015

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

121-129

Kód UT WoS článku

000366784500017

EID výsledku v databázi Scopus

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