Chitin and chitosan from Brazilian Atlantic Coast: Isolation, characterization and antibacterial activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900613" target="_blank" >RIV/00216275:25310/15:39900613 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/15:PU114525

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2015.06.027" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2015.06.027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2015.06.027" target="_blank" >10.1016/j.ijbiomac.2015.06.027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chitin and chitosan from Brazilian Atlantic Coast: Isolation, characterization and antibacterial activity

Popis výsledku v původním jazyce

Chitin and chitosan were obtained by chemical treatments of shrimp shells. Different particle sizes (50-1000 mu m) of the raw material were used to study their effect on size distribution, demineralization, deproteinization and deacetylation of chitin and chitosan isolation process. The particle size in the range of 800-1000 mu m was selected to isolate chitin, which was achieved by measuring nitrogen, protein, ash, and yield %. Hydrochloric acid (5%, v/v) was optimized in demineralization step to remove the minerals from the starting material. Aqueous solution of sodium hydroxide (5%, w/v) at 90 degrees C for (20h) was used in deproteinization step to remove the protein. Pure chitin was consequently impregnated into high concentration of sodium hydroxide (50%) for 3.5 h at 90 degrees C to remove the acetyl groups in order to form high pure chitosan. The degree of deacetylation (DDA) of chitosan was controlled and evaluated by different analytical tools. The chemical structure of chitin and chitosan was confirmed by elemental analysis, ATR-FTIR, H/C NMR, XRD, SEM, UV-Vis spectroscopy, TGA, and acid-base titration. The isolated chitin and chitosan from shrimp shell showed excellent antibacterial activity against Gram (-ve) bacteria (Escherichia coli) comparing with commercial biopolymers.

Název v anglickém jazyce

Chitin and chitosan from Brazilian Atlantic Coast: Isolation, characterization and antibacterial activity

Popis výsledku anglicky

Chitin and chitosan were obtained by chemical treatments of shrimp shells. Different particle sizes (50-1000 mu m) of the raw material were used to study their effect on size distribution, demineralization, deproteinization and deacetylation of chitin and chitosan isolation process. The particle size in the range of 800-1000 mu m was selected to isolate chitin, which was achieved by measuring nitrogen, protein, ash, and yield %. Hydrochloric acid (5%, v/v) was optimized in demineralization step to remove the minerals from the starting material. Aqueous solution of sodium hydroxide (5%, w/v) at 90 degrees C for (20h) was used in deproteinization step to remove the protein. Pure chitin was consequently impregnated into high concentration of sodium hydroxide (50%) for 3.5 h at 90 degrees C to remove the acetyl groups in order to form high pure chitosan. The degree of deacetylation (DDA) of chitosan was controlled and evaluated by different analytical tools. The chemical structure of chitin and chitosan was confirmed by elemental analysis, ATR-FTIR, H/C NMR, XRD, SEM, UV-Vis spectroscopy, TGA, and acid-base titration. The isolated chitin and chitosan from shrimp shell showed excellent antibacterial activity against Gram (-ve) bacteria (Escherichia coli) comparing with commercial biopolymers.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

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Svazek periodika

80

Číslo periodika v rámci svazku

September 2015

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

107-120

Kód UT WoS článku

000361251000013

EID výsledku v databázi Scopus

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