Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900647" target="_blank" >RIV/00216275:25310/15:39900647 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.mdpi.com/2227-9040/3/2/169" target="_blank" >http://www.mdpi.com/2227-9040/3/2/169</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/chemosensors3020169" target="_blank" >10.3390/chemosensors3020169</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

Popis výsledku v původním jazyce

An alcohol dehydrogenase-based biosensor was prepared and tested for its use to determine ethanol in beer. The biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide and immobilized with a biocatalytic layer containingthe enzyme. Function of the enzyme biosensor was tested in model ethanol samples, in which it showed a linear range of 15-120 g.L-1 with a detection limit of 3.3 g.L-1 (established as 3 sigma) and response time of 19 s. In a potential window from -0.2 to +0.45 V, interferences of both ascorbic and uric acids were negligible. Several types of marketed beers of Czech provenance were selected and subjected to measurements under optimized conditions but without any pretreatment of real samples. When compared with the reference method (gas chromatography), the results were in quite good agreement for beers of the pale lager type but higher contents of ethanol were indicated in the samples of dark lager beers.

Název v anglickém jazyce

Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

Popis výsledku anglicky

An alcohol dehydrogenase-based biosensor was prepared and tested for its use to determine ethanol in beer. The biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide and immobilized with a biocatalytic layer containingthe enzyme. Function of the enzyme biosensor was tested in model ethanol samples, in which it showed a linear range of 15-120 g.L-1 with a detection limit of 3.3 g.L-1 (established as 3 sigma) and response time of 19 s. In a potential window from -0.2 to +0.45 V, interferences of both ascorbic and uric acids were negligible. Several types of marketed beers of Czech provenance were selected and subjected to measurements under optimized conditions but without any pretreatment of real samples. When compared with the reference method (gas chromatography), the results were in quite good agreement for beers of the pale lager type but higher contents of ethanol were indicated in the samples of dark lager beers.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosensors

ISSN

2227-9040

e-ISSN

—

Svazek periodika

3

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

169-177

Kód UT WoS článku

000362073300004

EID výsledku v databázi Scopus

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