Determination of Branched Chain Fatty Acids in Plasma of Type 2 Diabetics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39901429" target="_blank" >RIV/00216275:25310/16:39901429 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Branched Chain Fatty Acids in Plasma of Type 2 Diabetics

Popis výsledku v původním jazyce

Insulin resistance in type 2 diabetic patients reduces activation of PPAR, which may lead to accumulation of branched chain fatty acids as well as saturated fatty acids. Natural sources of these fatty acids are dairy products. The aim of our study was to verify whether the accumulation of branched chain fatty acids takes place in type 2 diabetes and in positive case to specify the corresponding lipid fraction. 23 anonymized plasma samples of type 2 diabetic patients, which were subsequently divided by glycosidic haemoglobin levels into groups of 11 compensated and 12 decompensated; and plasma of 10 healthy blood donors were processed. At first the samples were divided into particular lipid classes using the thin layer chromatography. Then we set the content of individual fatty acids in all lipid classes using the gas chromatography. Results were calculated with statistical application SigmaStat 3.5. The most abundant branched chain fatty acid is 14-methylhexadecanoic acid. Statistically significant increase of this acid was found both in compensated diabetics (pLESS-THAN OR EQUAL TO0.001) and in the decompensated ones (pLESS-THAN OR EQUAL TO0.001) in comparison with controls. The 14-methylhexadecanoic acid was found in diacylglycerol fraction as well as in the free fatty acid fraction in compensated (p=0.008) and decompensated (p=0.007) diabetics. Increase of the content of branched chain fatty acids in diabetic patients was proved. Accumulation of branched chain fatty acids in diabetics raises a question on the precise influence of these fatty acids on the human organism.

Název v anglickém jazyce

Determination of Branched Chain Fatty Acids in Plasma of Type 2 Diabetics

Popis výsledku anglicky

Insulin resistance in type 2 diabetic patients reduces activation of PPAR, which may lead to accumulation of branched chain fatty acids as well as saturated fatty acids. Natural sources of these fatty acids are dairy products. The aim of our study was to verify whether the accumulation of branched chain fatty acids takes place in type 2 diabetes and in positive case to specify the corresponding lipid fraction. 23 anonymized plasma samples of type 2 diabetic patients, which were subsequently divided by glycosidic haemoglobin levels into groups of 11 compensated and 12 decompensated; and plasma of 10 healthy blood donors were processed. At first the samples were divided into particular lipid classes using the thin layer chromatography. Then we set the content of individual fatty acids in all lipid classes using the gas chromatography. Results were calculated with statistical application SigmaStat 3.5. The most abundant branched chain fatty acid is 14-methylhexadecanoic acid. Statistically significant increase of this acid was found both in compensated diabetics (pLESS-THAN OR EQUAL TO0.001) and in the decompensated ones (pLESS-THAN OR EQUAL TO0.001) in comparison with controls. The 14-methylhexadecanoic acid was found in diacylglycerol fraction as well as in the free fatty acid fraction in compensated (p=0.008) and decompensated (p=0.007) diabetics. Increase of the content of branched chain fatty acids in diabetic patients was proved. Accumulation of branched chain fatty acids in diabetics raises a question on the precise influence of these fatty acids on the human organism.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

ISSN

1211-5541

e-ISSN

—

Svazek periodika

22

Číslo periodika v rámci svazku

květen

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

57-66

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—