Retention of small molecules on polymethacrylate monolithic capillary columns

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39910846" target="_blank" >RIV/00216275:25310/17:39910846 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Retention of small molecules on polymethacrylate monolithic capillary columns

Popis výsledku v původním jazyce

In this paper, the concentration of N-isopropylacrylamide in the polymerization mixture has been variedto prepare several polymethacrylate monolithic capillary columns. Polymer monoliths combining N-isopropylacrylamide with zwitterion monomer, as well as various dimethacrylate crosslinking monomershave been prepared and characterized. Uracil, thiourea, phenol, toluene, ethylbenzene, propylbenzene,and butylbenzene have been used to characterize retention of prepared capillary columns in the mobilephases with 40–95% of acetonitrile and at working temperatures ranging from 25 to 60◦C. By an optimiza-tion of six-parameter polynomial models we have found that the retention of small molecules is affectedmainly by the concentration of the acetonitrile in the mobile phase with very low contribution of work-ing temperature and combined effect of acetonitrile concentration and temperature. Concentration ofthe mobile phase controlled also enthalpy of the retention. On the other hand, entropic contribution wasalmost insensitive to the change of the mobile phase composition, especially for mobile phases containingmore than 60% of acetonitrile.

Název v anglickém jazyce

Retention of small molecules on polymethacrylate monolithic capillary columns

Popis výsledku anglicky

In this paper, the concentration of N-isopropylacrylamide in the polymerization mixture has been variedto prepare several polymethacrylate monolithic capillary columns. Polymer monoliths combining N-isopropylacrylamide with zwitterion monomer, as well as various dimethacrylate crosslinking monomershave been prepared and characterized. Uracil, thiourea, phenol, toluene, ethylbenzene, propylbenzene,and butylbenzene have been used to characterize retention of prepared capillary columns in the mobilephases with 40–95% of acetonitrile and at working temperatures ranging from 25 to 60◦C. By an optimiza-tion of six-parameter polynomial models we have found that the retention of small molecules is affectedmainly by the concentration of the acetonitrile in the mobile phase with very low contribution of work-ing temperature and combined effect of acetonitrile concentration and temperature. Concentration ofthe mobile phase controlled also enthalpy of the retention. On the other hand, entropic contribution wasalmost insensitive to the change of the mobile phase composition, especially for mobile phases containingmore than 60% of acetonitrile.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1488

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

85-92

Kód UT WoS článku

000395853700010

EID výsledku v databázi Scopus

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