Voltammetric determination of cholecalciferol at glassy carbon electrode performed in water-ethanol mixture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39912547" target="_blank" >RIV/00216275:25310/18:39912547 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/viewFile/889/pdf/" target="_blank" >http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/viewFile/889/pdf/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5219/889" target="_blank" >10.5219/889</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Voltammetric determination of cholecalciferol at glassy carbon electrode performed in water-ethanol mixture

Popis výsledku v původním jazyce

To confirm or disprove previous hypotheses, cyclic voltammetry of 0.5 mM cholecalciferol (vitamin D3) at glassy carbon electrode (GCE) and platinum disk electrode (PtE) in pure acetonitrile and water-ethanol mixture at 50 mV·s-1 has been used to investigate the oxidation mechanism. The oxidation occurs in two one-electrone steps. According to calculation of the highest electron density in cholecalciferol molecule which is evidently delocalized over carbon atoms of the three conjugated double bonds (C19, C10, C5-C8) points to part of the molecule involved in oxidation processes. An oxidation peak (at +0.925 V vs. Ag/AgCl) was used to develop direct voltammetric method based on differential pulse voltammetry for the vitamin D3 determination at GCE performed in 40% ethanol containing 0.1 M LiClO4. Under optimization of analytical procedure, it was found that a composition of the supporting electrolyte used significantly affects a current response of oxidation peak obtained. Satisfactory sensitivity was achieved in the 1:1 water-ethanol mixture containing 0.05 M lithium perchlorate as as supporting electrolyte. The linear range for vitamin D3 determination was 2.4 × 10−6 – 3.5 × 10−4 M with the detection limit of 8.0 × 10−7 M. This work demonstrates a fact that the GCE is suitable electroanalytical device for analysis of various food supplements and medicaments.

Název v anglickém jazyce

Voltammetric determination of cholecalciferol at glassy carbon electrode performed in water-ethanol mixture

Popis výsledku anglicky

To confirm or disprove previous hypotheses, cyclic voltammetry of 0.5 mM cholecalciferol (vitamin D3) at glassy carbon electrode (GCE) and platinum disk electrode (PtE) in pure acetonitrile and water-ethanol mixture at 50 mV·s-1 has been used to investigate the oxidation mechanism. The oxidation occurs in two one-electrone steps. According to calculation of the highest electron density in cholecalciferol molecule which is evidently delocalized over carbon atoms of the three conjugated double bonds (C19, C10, C5-C8) points to part of the molecule involved in oxidation processes. An oxidation peak (at +0.925 V vs. Ag/AgCl) was used to develop direct voltammetric method based on differential pulse voltammetry for the vitamin D3 determination at GCE performed in 40% ethanol containing 0.1 M LiClO4. Under optimization of analytical procedure, it was found that a composition of the supporting electrolyte used significantly affects a current response of oxidation peak obtained. Satisfactory sensitivity was achieved in the 1:1 water-ethanol mixture containing 0.05 M lithium perchlorate as as supporting electrolyte. The linear range for vitamin D3 determination was 2.4 × 10−6 – 3.5 × 10−4 M with the detection limit of 8.0 × 10−7 M. This work demonstrates a fact that the GCE is suitable electroanalytical device for analysis of various food supplements and medicaments.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Potravinarstvo

ISSN

1338-0230

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

7

Strana od-do

166-172

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85043483452