Determination of amines using HPLC with fluorescence detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39913553" target="_blank" >RIV/00216275:25310/18:39913553 - isvavai.cz</a>
Výsledek na webu
—
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of amines using HPLC with fluorescence detection
Popis výsledku v původním jazyce
A simple and sensitive method for the determination of free aliphatic and aromatic amines using derivatives agents (dansyl chloride, 9-fluorenylmethyl chloroformate and 4-Chloro-7-nitrobenzofurazan) as a labelling reagent by high-performance liq-uid chromatography with fluorescence detection (HPLC-FLD) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. A fluorescence derivatization - high-performance liquid chromatography (HPLC) method, which enables the femtomole-level detection of analytes, is a powerful tool for the analysis with high sensitivity and selectivity. In recent decades, improvised explosive devices (IEDs) have become an increasing topic of public concern, with high-profile incidents such as the Bali (2002), London transport (2005), Boston Marathon (2013) and Brussels (2016) bombings garnering mass international attention.[1] Although the majority of media coverage has fo-cused on large-scale incidents, there has also been a rise in explosive incidents in-volving smaller devices such as pipe bombs. This is particularly the case in the Unit-ed States, where pipe bombs are considered to make up the vast majority of IED en-counter. These devices can be easily constructed from everyday materials.[2] The mixture nitromethane with amines is applicable for this. Determination of resi-dues after an explosion is important. This method (HPLC-FLD) could be useful for their determination. The proposed method is sensitive and reproducible for the de-termination of aliphatic and aromatic amines.
Název v anglickém jazyce
Determination of amines using HPLC with fluorescence detection
Popis výsledku anglicky
A simple and sensitive method for the determination of free aliphatic and aromatic amines using derivatives agents (dansyl chloride, 9-fluorenylmethyl chloroformate and 4-Chloro-7-nitrobenzofurazan) as a labelling reagent by high-performance liq-uid chromatography with fluorescence detection (HPLC-FLD) has been developed. Derivatization conditions including reagent concentration, buffer pH, reaction time and temperature were optimized. A fluorescence derivatization - high-performance liquid chromatography (HPLC) method, which enables the femtomole-level detection of analytes, is a powerful tool for the analysis with high sensitivity and selectivity. In recent decades, improvised explosive devices (IEDs) have become an increasing topic of public concern, with high-profile incidents such as the Bali (2002), London transport (2005), Boston Marathon (2013) and Brussels (2016) bombings garnering mass international attention.[1] Although the majority of media coverage has fo-cused on large-scale incidents, there has also been a rise in explosive incidents in-volving smaller devices such as pipe bombs. This is particularly the case in the Unit-ed States, where pipe bombs are considered to make up the vast majority of IED en-counter. These devices can be easily constructed from everyday materials.[2] The mixture nitromethane with amines is applicable for this. Determination of resi-dues after an explosion is important. This method (HPLC-FLD) could be useful for their determination. The proposed method is sensitive and reproducible for the de-termination of aliphatic and aromatic amines.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/VI20152020004" target="_blank" >VI20152020004: Identifikace reziduí improvizovaných výbušnin fyzikálně-chemickými analytickými metodami za reálných podmínek po výbuchu</a><br>
Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Proceedings of the 21st Seminar on New Trends in Research of Energetic Materials
ISBN
978-80-7560-136-0
ISSN
—
e-ISSN
neuvedeno
Počet stran výsledku
5
Strana od-do
533-537
Název nakladatele
Univerzita Pardubice
Místo vydání
Pardubice
Místo konání akce
Pardubice
Datum konání akce
18. 4. 2018
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
—