Mitochondria in Biodosimetry: Flow Cytometry Assessment in Vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25520%2F22%3A39918891" target="_blank" >RIV/00216275:25520/22:39918891 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/rpd/article-abstract/198/9-11/521/6673013" target="_blank" >https://academic.oup.com/rpd/article-abstract/198/9-11/521/6673013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/rpd/ncac092" target="_blank" >10.1093/rpd/ncac092</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mitochondria in Biodosimetry: Flow Cytometry Assessment in Vitro

Popis výsledku v původním jazyce

The JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. The probe was tested in vitro on two established cell lines and peripheral porcine blood lymphocytes after gamma irradiation (IR) to assess its potential in biodosimetric evaluation. In brief, we stained irradiated and non-irradiated cells with the JC-1 dye to determine the existing changes in mitochondrial membrane potential and monitor cell health through flow cytometry. The stage of injury in these cells was evaluated through an irradiated versus non-irradiated ratio (IVNIR), comparing the relative proportion of polarised cells containing red JC-1 aggregates. We observed a decreasing IVNIR as the radiation dose increased (i.e. 0.5; 1; 2; 4; 6; 8 and 10 Gy), performing the analysis at 4, 8 and 24 h after IR in all the tested cells. The results from the JC1-dye test showed that CD4 T lymphocytes were more sensitive to irradiation than other subpopulations.

Název v anglickém jazyce

Mitochondria in Biodosimetry: Flow Cytometry Assessment in Vitro

Popis výsledku anglicky

The JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health. The probe was tested in vitro on two established cell lines and peripheral porcine blood lymphocytes after gamma irradiation (IR) to assess its potential in biodosimetric evaluation. In brief, we stained irradiated and non-irradiated cells with the JC-1 dye to determine the existing changes in mitochondrial membrane potential and monitor cell health through flow cytometry. The stage of injury in these cells was evaluated through an irradiated versus non-irradiated ratio (IVNIR), comparing the relative proportion of polarised cells containing red JC-1 aggregates. We observed a decreasing IVNIR as the radiation dose increased (i.e. 0.5; 1; 2; 4; 6; 8 and 10 Gy), performing the analysis at 4, 8 and 24 h after IR in all the tested cells. The results from the JC1-dye test showed that CD4 T lymphocytes were more sensitive to irradiation than other subpopulations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Radiation Protection Dosimetry

ISSN

0144-8420

e-ISSN

1742-3406

Svazek periodika

198

Číslo periodika v rámci svazku

9-11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

521-526

Kód UT WoS článku

000844405700005

EID výsledku v databázi Scopus

2-s2.0-85137124613