Coherence-controlled holographic microscopy for live-cell quantitative phase imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26210%2F15%3APU114546" target="_blank" >RIV/00216305:26210/15:PU114546 - isvavai.cz</a>

Výsledek na webu

<a href="http://spie.org/Publications/Proceedings/Paper/10.1117/12.2080128" target="_blank" >http://spie.org/Publications/Proceedings/Paper/10.1117/12.2080128</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2080128" target="_blank" >10.1117/12.2080128</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging

Popis výsledku v původním jazyce

In this paper we present coherence-controlled holographic microscopy (CCHM) and various examples of observations of living cells including combination of CCHM with fluorescence microscopy. CCHM is a novel technique of quantitative phase imaging (QPI). Itis based on grating off-axis interferometer, which is fully adapted for the use of incoherent illumination. This enables high-quality QPI free from speckles and parasitic interferences and lateral resolution of classical widefield microscopes. Label-free nature of QPI makes CCHM a useful tool for long-term observations of living cells. Moreover, coherence-gating effect induced by the use of incoherent illumination enables QPI of cells even in scattering media. Combination of CCHM with common imaging techniques brings the possibility to exploit advantages of QPI while simultaneously identifying the observed structures or processes by well-established imaging methods. We used CCHM for investigation of general parameters of cell life cycl

Název v anglickém jazyce

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging

Popis výsledku anglicky

In this paper we present coherence-controlled holographic microscopy (CCHM) and various examples of observations of living cells including combination of CCHM with fluorescence microscopy. CCHM is a novel technique of quantitative phase imaging (QPI). Itis based on grating off-axis interferometer, which is fully adapted for the use of incoherent illumination. This enables high-quality QPI free from speckles and parasitic interferences and lateral resolution of classical widefield microscopes. Label-free nature of QPI makes CCHM a useful tool for long-term observations of living cells. Moreover, coherence-gating effect induced by the use of incoherent illumination enables QPI of cells even in scattering media. Combination of CCHM with common imaging techniques brings the possibility to exploit advantages of QPI while simultaneously identifying the observed structures or processes by well-established imaging methods. We used CCHM for investigation of general parameters of cell life cycl

Druh

D - Stať ve sborníku

CEP obor

BH - Optika, masery a lasery

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Quantitative Phase Imaging

ISBN

978-1-62841-426-4

ISSN

—

e-ISSN

—

Počet stran výsledku

4

Strana od-do

"93360K-1"-"93360K-4"

Název nakladatele

SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

Místo vydání

San Francisco, CA

Místo konání akce

San Francisco

Datum konání akce

7. 2. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000354170000008