Identification and Characterization of Sol Operon in Clostridium Pasteurianum NRRL B-598 Genome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F14%3APU110166" target="_blank" >RIV/00216305:26220/14:PU110166 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.13140/2.1.3688.6402" target="_blank" >http://dx.doi.org/10.13140/2.1.3688.6402</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.13140/2.1.3688.6402" target="_blank" >10.13140/2.1.3688.6402</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification and Characterization of Sol Operon in Clostridium Pasteurianum NRRL B-598 Genome

Popis výsledku v původním jazyce

Bacteria that belong to the genus Clostridium are a diverse group of rod-shaped anaerobes including a large number of solvent-producing species. However, these species are widely studied due to their utilization in biotechnology, the strain Clostridium pasteurianum NRRL B-598 has been used in only a few studies. This strain is characterized by its genetic stability and oxygen insensitivity. In this work, we present a bioinformatics analysis (mining) of the genome we sequenced recently. The strain NRRL B-598 genome was assembled to the contig level and annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). However, automatic annotation predicted 5,547 genes; it frequently failed in gene accurate denomination and further manual analysis was needed. Using BLAST homology search, we have determined a position of sol operon consisting of 4 genes: ald (aldehyde dehydrogenase), ctfA (CoA transferase subunit A), ctfB (CoA transferase subunit B), and adc (acetoacetate de

Název v anglickém jazyce

Identification and Characterization of Sol Operon in Clostridium Pasteurianum NRRL B-598 Genome

Popis výsledku anglicky

Bacteria that belong to the genus Clostridium are a diverse group of rod-shaped anaerobes including a large number of solvent-producing species. However, these species are widely studied due to their utilization in biotechnology, the strain Clostridium pasteurianum NRRL B-598 has been used in only a few studies. This strain is characterized by its genetic stability and oxygen insensitivity. In this work, we present a bioinformatics analysis (mining) of the genome we sequenced recently. The strain NRRL B-598 genome was assembled to the contig level and annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). However, automatic annotation predicted 5,547 genes; it frequently failed in gene accurate denomination and further manual analysis was needed. Using BLAST homology search, we have determined a position of sol operon consisting of 4 genes: ald (aldehyde dehydrogenase), ctfA (CoA transferase subunit A), ctfB (CoA transferase subunit B), and adc (acetoacetate de

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/GAP102%2F11%2F1068" target="_blank" >GAP102/11/1068: Nano-elektro-bio-nástroje pro biochemické a molekulárně-biologické studie eukaryotických buněk (NanoBioTECell)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

2. mezinárodní chemicko-technologická konference, SBORNÍK ABSTRAKT A PLNÝCH TEXTŮ

ISBN

978-80-86238-61-6

ISSN

—

e-ISSN

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Počet stran výsledku

5

Strana od-do

1-5

Název nakladatele

Neuveden

Místo vydání

Mikulov

Místo konání akce

Mikulov

Datum konání akce

7. 4. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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