Characterization of antibiotic resistance gene abundance and microbiota composition in feces of organic and conventional pigs from four EU countries

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F15%3APU114537" target="_blank" >RIV/00216305:26220/15:PU114537 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0132892" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0132892</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0132892" target="_blank" >10.1371/journal.pone.0132892</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of antibiotic resistance gene abundance and microbiota composition in feces of organic and conventional pigs from four EU countries

Popis výsledku v původním jazyce

One of the recent trends in animal production is the re-appearance of organic farming. The interest of wide public in organic farming is affected by interest in animal welfare and lower use of antibiotics in organic than in conventional farming systems.On the other hand, the ambition to provide the animals with a more natural life implies their increased exposure to environmental sources of different microorganisms including pathogens. To address these concerns, we determined the abundance of antibiotic resistance and diversity in fecal microbiota in slaughtered pigs kept under conventional and organic farming systems in Sweden, Denmark, France and Italy. Abundance of sul1, sul2, strA, tet(A), tet(B) and cat antibiotic resistance genes was determinedby real time PCR genes in 468 samples and the faecal microbiota diversity was characterised in selected 48 samples by pyrosequencing of V3/V4 regions of 16S rRNA. Unlike expectation, there were no significant differences between the abund

Název v anglickém jazyce

Characterization of antibiotic resistance gene abundance and microbiota composition in feces of organic and conventional pigs from four EU countries

Popis výsledku anglicky

One of the recent trends in animal production is the re-appearance of organic farming. The interest of wide public in organic farming is affected by interest in animal welfare and lower use of antibiotics in organic than in conventional farming systems.On the other hand, the ambition to provide the animals with a more natural life implies their increased exposure to environmental sources of different microorganisms including pathogens. To address these concerns, we determined the abundance of antibiotic resistance and diversity in fecal microbiota in slaughtered pigs kept under conventional and organic farming systems in Sweden, Denmark, France and Italy. Abundance of sul1, sul2, strA, tet(A), tet(B) and cat antibiotic resistance genes was determinedby real time PCR genes in 468 samples and the faecal microbiota diversity was characterised in selected 48 samples by pyrosequencing of V3/V4 regions of 16S rRNA. Unlike expectation, there were no significant differences between the abund

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLOS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

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EID výsledku v databázi Scopus

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