An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F16%3APU120311" target="_blank" >RIV/00216305:26220/16:PU120311 - isvavai.cz</a>

Výsledek na webu

<a href="https://ieeexplore.ieee.org/document/7868755" target="_blank" >https://ieeexplore.ieee.org/document/7868755</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

Popis výsledku v původním jazyce

Neonatal myocytes are a widely used model for modern in-vitro pharmacological screening and tissue engineering of cardiac tissue. The recording of neonatal myocyte electrophysiology in real time has still not been ideally optimised, however. The aim of this study was to evaluate the impact of using different substrates in culture (MEA) chambers on the surviving and functional electrophysiological activity of the myocytes after the first five days after isolation. Neonatal cardiomyocytes were isolated from two day-old neonatal rats and plated on 12×12 ITO microelectrode chambers (Multi-Channel Systems) and cultured for five days. Four coating methods were used. The PDL + fibronectin treatment displayed the highest cardiomyocyte adhesion to the electrodes (20% of total cells in chamber after seeding) and developed contractible cells during the five days of pre-cultivation with a measurable electrical response. Nevertheless, the cells adhered to all surface-treated MEA chambers, creating typical cell morphology, but with different success rates.

Název v anglickém jazyce

An evaluation of different coatings for TiN microelectrode chambers used for neonatal cardiomyocytes

Popis výsledku anglicky

Neonatal myocytes are a widely used model for modern in-vitro pharmacological screening and tissue engineering of cardiac tissue. The recording of neonatal myocyte electrophysiology in real time has still not been ideally optimised, however. The aim of this study was to evaluate the impact of using different substrates in culture (MEA) chambers on the surviving and functional electrophysiological activity of the myocytes after the first five days after isolation. Neonatal cardiomyocytes were isolated from two day-old neonatal rats and plated on 12×12 ITO microelectrode chambers (Multi-Channel Systems) and cultured for five days. Four coating methods were used. The PDL + fibronectin treatment displayed the highest cardiomyocyte adhesion to the electrodes (20% of total cells in chamber after seeding) and developed contractible cells during the five days of pre-cultivation with a measurable electrical response. Nevertheless, the cells adhered to all surface-treated MEA chambers, creating typical cell morphology, but with different success rates.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Computing in Cardiology 2016

ISBN

978-1-5090-0684-7

ISSN

0276-6574

e-ISSN

—

Počet stran výsledku

4

Strana od-do

365-368

Název nakladatele

Computing in Cardiology

Místo vydání

Vancouver, Canada

Místo konání akce

Vancouver

Datum konání akce

11. 9. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000405710400092