Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F18%3APU129244" target="_blank" >RIV/00216305:26220/18:PU129244 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.22489/CinC.2018.103" target="_blank" >http://dx.doi.org/10.22489/CinC.2018.103</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22489/CinC.2018.103" target="_blank" >10.22489/CinC.2018.103</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

Popis výsledku v původním jazyce

In current cell tissue engineering and cardiology, beating models with behavior and properties similar to humans are necessary. The cardiac muscle HL-1 cells, whose genes have a parent with adult atrial myocytes, serve as a suitable model. HL-1 cells were seeded on a 120-electrode microelectrode (MEA) chamber and transient transfection of Accelerated Sensor of Action Potentials 1 (ASAP1), a genetically encoded voltage indicator, was performed. Simultaneous electrical and optical recording of cardiac cell culture electrical activity was made when the cells started to produce spontaneous action potentials. Recording synchronization was made using TTL pulses. Results showed that HL-1 cells start to produce asynchronous spontaneous action potentials (-375±10 µV) when reaching 90% MEA chamber confluency and which become periodical addition of extracellular calcium (-501±14 µV, 2.1±1.0 Hz). The ASAP1’s fluorescent response reached up to 21±5% ΔF/F. The time constant between detection of electrical and fluorescent response (τe/o) was determined as 12±5 ms.

Název v anglickém jazyce

Simultaneous electrical and fluorescence recording of HL-1 cells’ electrical activity in response to extracellular calcium stimulation

Popis výsledku anglicky

In current cell tissue engineering and cardiology, beating models with behavior and properties similar to humans are necessary. The cardiac muscle HL-1 cells, whose genes have a parent with adult atrial myocytes, serve as a suitable model. HL-1 cells were seeded on a 120-electrode microelectrode (MEA) chamber and transient transfection of Accelerated Sensor of Action Potentials 1 (ASAP1), a genetically encoded voltage indicator, was performed. Simultaneous electrical and optical recording of cardiac cell culture electrical activity was made when the cells started to produce spontaneous action potentials. Recording synchronization was made using TTL pulses. Results showed that HL-1 cells start to produce asynchronous spontaneous action potentials (-375±10 µV) when reaching 90% MEA chamber confluency and which become periodical addition of extracellular calcium (-501±14 µV, 2.1±1.0 Hz). The ASAP1’s fluorescent response reached up to 21±5% ΔF/F. The time constant between detection of electrical and fluorescent response (τe/o) was determined as 12±5 ms.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Computing in Cardiology 2018

ISBN

—

ISSN

2325-887X

e-ISSN

—

Počet stran výsledku

4

Strana od-do

1-4

Název nakladatele

Computing in Cardiology

Místo vydání

Maastricht, Netherlands

Místo konání akce

Maastricht

Datum konání akce

23. 9. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000482598700162