Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models

Popis výsledku v původním jazyce

Voltage-sensitive dyes (VSD's) can be used in touch-less recording of action potentials from animal hearts. VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. This optical response is sufficiently fast to detect transient potential changes in excitable cardiac cells. Two animal models are employed in our laboratory to record monophasic action potentials using VSD in isolated hearts perfused accorrding to Langendorf - guinea pig and rabbit. The hearts of both species undergo the same isolation and loading procedures. Briefly, the spontaneously beating heart is mounted on Langendorf set and perfused with constant perfusion pressure with Krebs-Henseleit solution (37°C, 1,25mM Ca2+). After control period (20min), the heart is loaded with di-4-ANEPPS (2uM) diluted in perfusion solution (25 min) and then washed-out with dye-free perfusion solution (25 min). It has been observed that durin

Název v anglickém jazyce

Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models

Popis výsledku anglicky

Voltage-sensitive dyes (VSD's) can be used in touch-less recording of action potentials from animal hearts. VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. This optical response is sufficiently fast to detect transient potential changes in excitable cardiac cells. Two animal models are employed in our laboratory to record monophasic action potentials using VSD in isolated hearts perfused accorrding to Langendorf - guinea pig and rabbit. The hearts of both species undergo the same isolation and loading procedures. Briefly, the spontaneously beating heart is mounted on Langendorf set and perfused with constant perfusion pressure with Krebs-Henseleit solution (37°C, 1,25mM Ca2+). After control period (20min), the heart is loaded with di-4-ANEPPS (2uM) diluted in perfusion solution (25 min) and then washed-out with dye-free perfusion solution (25 min). It has been observed that durin

Druh

D - Stať ve sborníku

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Computers in Cardiology

ISBN

0-7803-8894-1

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

1-4

Název nakladatele

IEEE

Místo vydání

Francie

Místo konání akce

Lyon

Datum konání akce

25. 9. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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