Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F17%3APU124924" target="_blank" >RIV/00216305:26220/17:PU124924 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-017-2038-1" target="_blank" >10.1007/s00706-017-2038-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

Popis výsledku v původním jazyce

The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within approximate to10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.

Název v anglickém jazyce

Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

Popis výsledku anglicky

The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within approximate to10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie

ISSN

0026-9247

e-ISSN

1434-4475

Svazek periodika

148

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

9

Strana od-do

1901-1909

Kód UT WoS článku

000413626000002

EID výsledku v databázi Scopus

2-s2.0-85029548548