Separation and detection of bioconjugated QDs in microfluidic devices using capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F15%3APU119551" target="_blank" >RIV/00216305:26620/15:PU119551 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Separation and detection of bioconjugated QDs in microfluidic devices using capillary electrophoresis

Popis výsledku v původním jazyce

Semiconductor nanocrystals, also known as quantum dots (QDs), are nanoscale inorganic particles, and have been attract a lot of attention due to their unique optic and electronic properties. One of the most important characteristic is their bioconjugation with different biological materials such as protein, peptides and nucleic acid. Due to very diverse and numerous applications, it is really important to make a tool for precise and controlled detection of QDs. In this study, we investigate on-a-chip detection and separation of QDs. Separation is done by capillary electrophoresis using high voltage and as a carrier fluid, different buffers. Detection is done using UV filter and photodetector including high voltage photomultiplier. In first step mold, microchannels width of 50 µm was made using silicon wafer and photolithography. Next, mixture of the PDMS was completely degassed and pours onto the wafer mold to create transparent, flexible chip. Our first results showed that this method can be used to

Název v anglickém jazyce

Separation and detection of bioconjugated QDs in microfluidic devices using capillary electrophoresis

Popis výsledku anglicky

Semiconductor nanocrystals, also known as quantum dots (QDs), are nanoscale inorganic particles, and have been attract a lot of attention due to their unique optic and electronic properties. One of the most important characteristic is their bioconjugation with different biological materials such as protein, peptides and nucleic acid. Due to very diverse and numerous applications, it is really important to make a tool for precise and controlled detection of QDs. In this study, we investigate on-a-chip detection and separation of QDs. Separation is done by capillary electrophoresis using high voltage and as a carrier fluid, different buffers. Detection is done using UV filter and photodetector including high voltage photomultiplier. In first step mold, microchannels width of 50 µm was made using silicon wafer and photolithography. Next, mixture of the PDMS was completely degassed and pours onto the wafer mold to create transparent, flexible chip. Our first results showed that this method can be used to

Druh

O - Ostatní výsledky

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů