LAMP-on-a-Chip: Revising Microfluidic Platforms for Loop-Mediated DNA Amplification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F19%3APU130656" target="_blank" >RIV/00216305:26220/19:PU130656 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.trac.2019.01.015" target="_blank" >http://dx.doi.org/10.1016/j.trac.2019.01.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.trac.2019.01.015" target="_blank" >10.1016/j.trac.2019.01.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

LAMP-on-a-Chip: Revising Microfluidic Platforms for Loop-Mediated DNA Amplification

Popis výsledku v původním jazyce

Nucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is less demanding in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.

Název v anglickém jazyce

LAMP-on-a-Chip: Revising Microfluidic Platforms for Loop-Mediated DNA Amplification

Popis výsledku anglicky

Nucleic acid amplification for the detection of infectious diseases, food pathogens, or assessment of genetic disorders require a laboratory setting with specialized equipment and technical expertise. Isothermal deoxyribonucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP), exhibit characteristics ideal for point-of-care (POC) applications, since their instrumentation is less demanding in comparison with the standard method of polymerase chain reaction. Other key advantages of LAMP are robustness and the production of pyrophosphate in the presence of the target gene, enabling to detect the reaction products using the naked eye. Polymerase inhibitors, presented in clinical samples, do not affect the amplification process, making LAMP suitable for a simple sample-to-answer diagnostic systems with simplified sample preparation. In this review, we discuss the trends in miniaturized LAMP techniques, such as microfluidic, paper-based, and digital with their advantages and disadvantages, especially for POC applications alongside our opinion of the future development of miniaturized LAMP.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

ISSN

0165-9936

e-ISSN

1879-3142

Svazek periodika

neuveden

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

44-53

Kód UT WoS článku

000461894400005

EID výsledku v databázi Scopus

2-s2.0-85061142460