Alternate current high voltage pulses induce apoptotic death in myocardium by irreversible electroporation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F19%3APU133980" target="_blank" >RIV/00216305:26220/19:PU133980 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Alternate current high voltage pulses induce apoptotic death in myocardium by irreversible electroporation

Popis výsledku v původním jazyce

Background: Irreversible electroporation (IRE) holds the promise to revolutionize catheter ablation of arrhythmia, overcoming the limits of thermal-based therapies. A careful combination of pulse parameters, safety conditions and knowledge of cell death mechanism must be pursued to maximize the benefits of the envisioned therapies. Aims: Identify a combination of parameters to induce complete local cell death. Prove the feasibility, efficacy and safety of AC-IRE ablation. Characterize the cellular and tissue damages induced by the AC-IRE application. Methods: Three study models are employed. In the first one, a cuvette-based system is used to calibrate the optimal pair of electric field-number of pulses to induce complete cell death. In the second one, a microelectrode array (MEA) system is used in combination with calcium imaging to identify electrical and ionic changes in cardiac cells affected by the identified pulse combination. In the third one, a swine model was used to study underwent AC-IRE ablation in left atrium (pulmonary vein ostia, chamber walls) and intraventricular septum.

Název v anglickém jazyce

Alternate current high voltage pulses induce apoptotic death in myocardium by irreversible electroporation

Popis výsledku anglicky

Background: Irreversible electroporation (IRE) holds the promise to revolutionize catheter ablation of arrhythmia, overcoming the limits of thermal-based therapies. A careful combination of pulse parameters, safety conditions and knowledge of cell death mechanism must be pursued to maximize the benefits of the envisioned therapies. Aims: Identify a combination of parameters to induce complete local cell death. Prove the feasibility, efficacy and safety of AC-IRE ablation. Characterize the cellular and tissue damages induced by the AC-IRE application. Methods: Three study models are employed. In the first one, a cuvette-based system is used to calibrate the optimal pair of electric field-number of pulses to induce complete cell death. In the second one, a microelectrode array (MEA) system is used in combination with calcium imaging to identify electrical and ionic changes in cardiac cells affected by the identified pulse combination. In the third one, a swine model was used to study underwent AC-IRE ablation in left atrium (pulmonary vein ostia, chamber walls) and intraventricular septum.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30502 - Other medical science

Projekt

<a href="/cs/project/LO1210" target="_blank" >LO1210: Energie v podmínkách udržitelného rozvoje (EN-PUR)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

3rd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food and Environmental Technologies

ISBN

978-2-913923-38-6

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

1-1

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Toulouse

Datum konání akce

3. 9. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—