Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca<SUP>2+</SUP> Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F24%3APU156032" target="_blank" >RIV/00216305:26220/24:PU156032 - isvavai.cz</a>

Výsledek na webu

<a href="https://ieeexplore.ieee.org/document/10473191" target="_blank" >https://ieeexplore.ieee.org/document/10473191</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1109/TBME.2024.3358240" target="_blank" >10.1109/TBME.2024.3358240</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca<SUP>2+</SUP> Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Popis výsledku v původním jazyce

Objective: This study aims to explore the potential of organic electrolytic photocapacitors (OEPCs), an innovative photovoltaic device, in mediating the activation of native voltage-gated Cav1.2 channels (I-Ca,I-L) in Guinea pig ventricular cardiomyocytes. Methods: Whole-cell patch-clamp recordings were employed to examine light-triggered OEPC mediated I-Ca,I-L activation, integrating the channel's kinetic properties into a multicompartment cell model to take intracellular ion concentrations into account. A multidomain model was additionally incorporated to evaluate effects of OEPC-mediated stimulation. The final model combines external stimulation, multicompartmental cell simulation, and a patch-clamp amplifier equivalent circuit to assess the impact on achievable intracellular voltage changes. Results: Light pulses activated I-Ca,I-L, with amplitudes similar to voltage-clamp activation and high sensitivity to the L-type Ca2+ channel blocker, nifedipine. Light-triggered I-Ca,I-L inactivation exhibited kinetic parameters comparable to voltage-induced inactivation. Conclusion: OEPC-mediated activation of I-Ca,I-L demonstrates their potential for nongenetic optical modulation of cellular physiology potentially paving the way for the development of innovative therapies in cardiovascular health. The integrated model proves the light-mediated activation of I-Ca,I-L and advances the understanding of the interplay between the patch-clamp amplifier and external stimulation devices. Significance: Treating cardiac conduction disorders by minimal-invasive means without genetic modifications could advance therapeutic approaches increasing patients' quality of life compared with conventional methods employing electronic devices.

Název v anglickém jazyce

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca<SUP>2+</SUP> Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Popis výsledku anglicky

Objective: This study aims to explore the potential of organic electrolytic photocapacitors (OEPCs), an innovative photovoltaic device, in mediating the activation of native voltage-gated Cav1.2 channels (I-Ca,I-L) in Guinea pig ventricular cardiomyocytes. Methods: Whole-cell patch-clamp recordings were employed to examine light-triggered OEPC mediated I-Ca,I-L activation, integrating the channel's kinetic properties into a multicompartment cell model to take intracellular ion concentrations into account. A multidomain model was additionally incorporated to evaluate effects of OEPC-mediated stimulation. The final model combines external stimulation, multicompartmental cell simulation, and a patch-clamp amplifier equivalent circuit to assess the impact on achievable intracellular voltage changes. Results: Light pulses activated I-Ca,I-L, with amplitudes similar to voltage-clamp activation and high sensitivity to the L-type Ca2+ channel blocker, nifedipine. Light-triggered I-Ca,I-L inactivation exhibited kinetic parameters comparable to voltage-induced inactivation. Conclusion: OEPC-mediated activation of I-Ca,I-L demonstrates their potential for nongenetic optical modulation of cellular physiology potentially paving the way for the development of innovative therapies in cardiovascular health. The integrated model proves the light-mediated activation of I-Ca,I-L and advances the understanding of the interplay between the patch-clamp amplifier and external stimulation devices. Significance: Treating cardiac conduction disorders by minimal-invasive means without genetic modifications could advance therapeutic approaches increasing patients' quality of life compared with conventional methods employing electronic devices.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20600 - Medical engineering

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING

ISSN

0018-9294

e-ISSN

1558-2531

Svazek periodika

71

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

1980-1992

Kód UT WoS článku

001230139500002

EID výsledku v databázi Scopus

2-s2.0-85188420084