Identification of a molecular gating determinant within the carboxy terminal region of Ca(v)3.3 T-type channels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00504415" target="_blank" >RIV/61388963:_____/19:00504415 - isvavai.cz</a>

Výsledek na webu

<a href="https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-019-0457-0" target="_blank" >https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-019-0457-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13041-019-0457-0" target="_blank" >10.1186/s13041-019-0457-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of a molecular gating determinant within the carboxy terminal region of Ca(v)3.3 T-type channels

Popis výsledku v původním jazyce

The physiological functions controlled by T-type channels are intrinsically dependent on their gating properties, and alteration of T-type channel activity is linked to several human disorders. Therefore, it is essential to develop a clear understanding of the structural determinants responsible for the unique gating features of T-type channels. Here, we have investigated the specific role of the carboxy terminal region by creating a series a deletion constructs expressed in tsA-201 cells and analyzing them by patch clamp electrophysiology. Our data reveal that the proximal region of the carboxy terminus contains a structural determinant essential for shaping several gating aspects of Ca(v)3.3 channels, including voltage-dependence of activation and inactivation, inactivation kinetics, and coupling between the voltage sensing and the pore opening of the channel. Altogether, our data are consistent with a model in which the carboxy terminus stabilizes the channel in a closed state.

Název v anglickém jazyce

Identification of a molecular gating determinant within the carboxy terminal region of Ca(v)3.3 T-type channels

Popis výsledku anglicky

The physiological functions controlled by T-type channels are intrinsically dependent on their gating properties, and alteration of T-type channel activity is linked to several human disorders. Therefore, it is essential to develop a clear understanding of the structural determinants responsible for the unique gating features of T-type channels. Here, we have investigated the specific role of the carboxy terminal region by creating a series a deletion constructs expressed in tsA-201 cells and analyzing them by patch clamp electrophysiology. Our data reveal that the proximal region of the carboxy terminus contains a structural determinant essential for shaping several gating aspects of Ca(v)3.3 channels, including voltage-dependence of activation and inactivation, inactivation kinetics, and coupling between the voltage sensing and the pore opening of the channel. Altogether, our data are consistent with a model in which the carboxy terminus stabilizes the channel in a closed state.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Brain

ISSN

1756-6606

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

Apr 8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

34

Kód UT WoS článku

000464218100002

EID výsledku v databázi Scopus

2-s2.0-85064120593