Selective inhibition of neuronal Cav3.3 T-type calcium channels by TAT-based channel peptide

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00525597" target="_blank" >RIV/61388963:_____/20:00525597 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/20:10413337

Výsledek na webu

<a href="https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-020-00636-y" target="_blank" >https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-020-00636-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13041-020-00636-y" target="_blank" >10.1186/s13041-020-00636-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Selective inhibition of neuronal Cav3.3 T-type calcium channels by TAT-based channel peptide

Popis výsledku v původním jazyce

Low-voltage-activated Cav3 calcium channels (T-type) play an essential role in the functioning of the nervous system where they support oscillatory activities that relie on several channel molecular determinants that shape their unique gating properties. In a previous study, we documented the important role of the carboxy proximal region in the functioning of Cav3.3 channels. Here, we explore the ability of a TAT-based cell penetrating peptide containing this carboxy proximal region (TAT-C3P) to modulate the activity of Cav3 channels. We show that chronic application of TAT-C3P on tsA-201 cells expressing Cav3 channels selectively inhibits Cav3.3 channels without affecting Cav3.1 and Cav3.2 channels. Therefore, the TAT-C3P peptide described in this study represents a new tool to address the specific physiological role of Cav3.3 channels, and to potentially enhance our understanding of Cav3.3 in disease.

Název v anglickém jazyce

Selective inhibition of neuronal Cav3.3 T-type calcium channels by TAT-based channel peptide

Popis výsledku anglicky

Low-voltage-activated Cav3 calcium channels (T-type) play an essential role in the functioning of the nervous system where they support oscillatory activities that relie on several channel molecular determinants that shape their unique gating properties. In a previous study, we documented the important role of the carboxy proximal region in the functioning of Cav3.3 channels. Here, we explore the ability of a TAT-based cell penetrating peptide containing this carboxy proximal region (TAT-C3P) to modulate the activity of Cav3 channels. We show that chronic application of TAT-C3P on tsA-201 cells expressing Cav3 channels selectively inhibits Cav3.3 channels without affecting Cav3.1 and Cav3.2 channels. Therefore, the TAT-C3P peptide described in this study represents a new tool to address the specific physiological role of Cav3.3 channels, and to potentially enhance our understanding of Cav3.3 in disease.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Brain

ISSN

1756-6606

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

Jun 19

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

3

Strana od-do

95

Kód UT WoS článku

000556296200001

EID výsledku v databázi Scopus

2-s2.0-85086752695