An alternative DSC approach to study hydration of hyaluronan

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F10%3APU87671" target="_blank" >RIV/00216305:26310/10:PU87671 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An alternative DSC approach to study hydration of hyaluronan

Popis výsledku v původním jazyce

Differential scanning calorimetry (DSC) was used to determine the number of water molecules in the hydration shell of hyaluronan of different molecular weights and counterions. First, traditional experiments including freezing/thawing of free water in semi-diluted solutions were carried out leading to the determination of melting enthalpy of freezable water. Non-freezing water was determined using extrapolation to zero enthalpy. For sodium hyaluronan within the molecular weight range between 100 and 740kDa the hydration shell was determined as 0.74 g g(-1) HYA. A larger hydration shell containing 0.84 and 0.82 g g(-1) HYA was determined for hyaluronan of 1390 kDa in its sodium and protonized form, respectively. Second, melting enthalpy of freezing water was further studied applying water vaporation experiments. Resulted plot of enthalpy vs concentration indicated an additional heat volution process which occurs at specific concentration and decreases the measured evaporation enthalpy.

Název v anglickém jazyce

An alternative DSC approach to study hydration of hyaluronan

Popis výsledku anglicky

Differential scanning calorimetry (DSC) was used to determine the number of water molecules in the hydration shell of hyaluronan of different molecular weights and counterions. First, traditional experiments including freezing/thawing of free water in semi-diluted solutions were carried out leading to the determination of melting enthalpy of freezable water. Non-freezing water was determined using extrapolation to zero enthalpy. For sodium hyaluronan within the molecular weight range between 100 and 740kDa the hydration shell was determined as 0.74 g g(-1) HYA. A larger hydration shell containing 0.84 and 0.82 g g(-1) HYA was determined for hyaluronan of 1390 kDa in its sodium and protonized form, respectively. Second, melting enthalpy of freezing water was further studied applying water vaporation experiments. Resulted plot of enthalpy vs concentration indicated an additional heat volution process which occurs at specific concentration and decreases the measured evaporation enthalpy.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CARBOHYDRATE POLYMERS

ISSN

0144-8617

e-ISSN

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Svazek periodika

82

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000280574600039

EID výsledku v databázi Scopus

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