Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F12%3APU101988" target="_blank" >RIV/00216305:26310/12:PU101988 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes

Popis výsledku v původním jazyce

Determination of organic acids is an important control steps in many biotechnological processes ? especially during microbial cultivation, must fermentation. Moreover, in many biotechnological products the final profile of the organic acids is an important factor. Determination of organic acids requires a fast and sensitive analytical method. Capillary zone electrophoresis (CZE) is a very suitable method for the determination of short-chain carboxylic acids, because they are easy to ionize. Further, CZEis an ecological and economical method, have low exigencies to quantity of samples. Method is fast, accurate and sensitive to low concentrations of analytes. Because short-chain aliphatic acids have not suitable chromophore, it is necessary to use indirect photometric detection for their determination. It was tested and optimized background electrolyte containing 3,5-dinitrobenzoic acid (10 ? 20 mM) as visualizing and buffering ion, set up to the pH 3,5 ? 4,0 (NaOH, TRIS). Cationic surf

Název v anglickém jazyce

Optimization of the capillary zone electrophoresis method with indirect photometry detection for monitoring of selected organic acid in biotechnological processes

Popis výsledku anglicky

Determination of organic acids is an important control steps in many biotechnological processes ? especially during microbial cultivation, must fermentation. Moreover, in many biotechnological products the final profile of the organic acids is an important factor. Determination of organic acids requires a fast and sensitive analytical method. Capillary zone electrophoresis (CZE) is a very suitable method for the determination of short-chain carboxylic acids, because they are easy to ionize. Further, CZEis an ecological and economical method, have low exigencies to quantity of samples. Method is fast, accurate and sensitive to low concentrations of analytes. Because short-chain aliphatic acids have not suitable chromophore, it is necessary to use indirect photometric detection for their determination. It was tested and optimized background electrolyte containing 3,5-dinitrobenzoic acid (10 ? 20 mM) as visualizing and buffering ion, set up to the pH 3,5 ? 4,0 (NaOH, TRIS). Cationic surf

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Studentská odborná konference Chemie je život 2012 - Sborník příspěvků.

ISBN

978-80-214-4644-1

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

271-275

Název nakladatele

Vysoké učení technické v Brně, Fakulta chemická,

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 12. 2012

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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