Magnetic driven separation techniques - DNA isolation from probiotic food samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU103835" target="_blank" >RIV/00216305:26310/13:PU103835 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic driven separation techniques - DNA isolation from probiotic food samples

Popis výsledku v původním jazyce

Magnetic driven separation techniques using magnetic solid particles are one of modern methods to speed up and facilitate the nucleic acids (DNA and RNA) separation and purification procedures. The problem of falsely negative results in PCR analysis of real food samples due to the presence of extracellular PCR inhibitors can be solved using solid phase systems adsorbing genomic DNA. In our work various types of magnetic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres, poly(2-hydroxyethyl methacrylate-co-etylene dimethacrylate) - P(HEMA-co-EDMA) and poly(glycidylmethacrylate) - (PGMA) covered by different content of carboxyl groups were used for the DNA isolation from crude cell lysates of food products such as yoghurts, probiotic dairy drinks and instant complete initial food. It was shown that tested microspheres are suitable for the PCR and qPCR ready DNA isolations from these types of food samples. The amount of isolated DNA correl

Název v anglickém jazyce

Magnetic driven separation techniques - DNA isolation from probiotic food samples

Popis výsledku anglicky

Magnetic driven separation techniques using magnetic solid particles are one of modern methods to speed up and facilitate the nucleic acids (DNA and RNA) separation and purification procedures. The problem of falsely negative results in PCR analysis of real food samples due to the presence of extracellular PCR inhibitors can be solved using solid phase systems adsorbing genomic DNA. In our work various types of magnetic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres, poly(2-hydroxyethyl methacrylate-co-etylene dimethacrylate) - P(HEMA-co-EDMA) and poly(glycidylmethacrylate) - (PGMA) covered by different content of carboxyl groups were used for the DNA isolation from crude cell lysates of food products such as yoghurts, probiotic dairy drinks and instant complete initial food. It was shown that tested microspheres are suitable for the PCR and qPCR ready DNA isolations from these types of food samples. The amount of isolated DNA correl

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GP13-32840P" target="_blank" >GP13-32840P: Vývoj metody pro studium enkapsulace magnetických nano- a mikročástic pomocí polymerázové řetězové reakce v reálném čase</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů