Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU106754" target="_blank" >RIV/00216305:26310/13:PU106754 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60193697:_____/13:#0000830

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11274-013-1410-5" target="_blank" >http://dx.doi.org/10.1007/s11274-013-1410-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11274-013-1410-5" target="_blank" >10.1007/s11274-013-1410-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil

Popis výsledku v původním jazyce

Using random chemical mutagenesis we obtained the mutant of Cupriavidus necator H16 which was capable of improved (about 35 %) production of poly(3-hydroxybuytrate) (PHB) compared to the wild-type strain. The mutant exhibited significantly enhanced specific activities of enzymes involved in oxidative stress response such as malic enzyme, NADP-dependent isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase. Probably, due to the activation of these enzymes, we also observed an increase of NADPH/NADP? ratio. It is likely that as a side effect of the increase of NADPH/NADP ratio the activity of PHBbiosynthetic pathwaywas enhanced,which supported the accumulation of PHB. Furthermore, the mutant was also able to incorporatepropionate into copolymer poly(3-hydroxybuytyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] more efficiently than the wild-type strain (Y3HV/prec = 0.17 and 0.29 for the wild-type strain and themutant, respectively)).We assume that it may be

Název v anglickém jazyce

Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil

Popis výsledku anglicky

Using random chemical mutagenesis we obtained the mutant of Cupriavidus necator H16 which was capable of improved (about 35 %) production of poly(3-hydroxybuytrate) (PHB) compared to the wild-type strain. The mutant exhibited significantly enhanced specific activities of enzymes involved in oxidative stress response such as malic enzyme, NADP-dependent isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase. Probably, due to the activation of these enzymes, we also observed an increase of NADPH/NADP? ratio. It is likely that as a side effect of the increase of NADPH/NADP ratio the activity of PHBbiosynthetic pathwaywas enhanced,which supported the accumulation of PHB. Furthermore, the mutant was also able to incorporatepropionate into copolymer poly(3-hydroxybuytyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] more efficiently than the wild-type strain (Y3HV/prec = 0.17 and 0.29 for the wild-type strain and themutant, respectively)).We assume that it may be

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/ED0012%2F01%2F01" target="_blank" >ED0012/01/01: Centra materiálového výzkumu na FCH VUT v Brně</a><br>

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

ISSN

0959-3993

e-ISSN

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Svazek periodika

29

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

2417-2428

Kód UT WoS článku

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EID výsledku v databázi Scopus

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