Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU112104" target="_blank" >RIV/00216305:26310/14:PU112104 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf" target="_blank" >http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

Popis výsledku v původním jazyce

The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used α amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for α amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 g∙L-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 g∙L 1.

Název v anglickém jazyce

Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

Popis výsledku anglicky

The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used α amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for α amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 g∙L-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 g∙L 1.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů