UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU107812" target="_blank" >RIV/00216305:26310/15:PU107812 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.foodchem.2014.08.072" target="_blank" >http://dx.doi.org/10.1016/j.foodchem.2014.08.072</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodchem.2014.08.072" target="_blank" >10.1016/j.foodchem.2014.08.072</a>

Jazyk výsledku

angličtina

Název v původním jazyce

UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed

Popis výsledku v původním jazyce

Alkaloids known as secondary metabolites are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs) comprising more than 350 individual heterocyclic compounds. The PAs present a serious health risk to human and livestock; hence there is a need for methods that allow determination of these dangerous plant toxins. In this study, fast, reliable and sensitive approach is proposed to identify and quantify PAs in feed samples. PAs including monocrotaline, senkirkine, senecionine, seneciphylline and retrorsine were determined by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Sample preparation is based on modified QuEChERS approach. Mean recovery, precision, matrix effects and limits of quantification (LOQ) were assessed for three matrices within the method validation. The presented method was tested on 41 various feed samples, where presence of PAs was expected.Roughages and feed for rabbits contained the highest levels of PAs, in general.

Název v anglickém jazyce

UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed

Popis výsledku anglicky

Alkaloids known as secondary metabolites are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs) comprising more than 350 individual heterocyclic compounds. The PAs present a serious health risk to human and livestock; hence there is a need for methods that allow determination of these dangerous plant toxins. In this study, fast, reliable and sensitive approach is proposed to identify and quantify PAs in feed samples. PAs including monocrotaline, senkirkine, senecionine, seneciphylline and retrorsine were determined by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Sample preparation is based on modified QuEChERS approach. Mean recovery, precision, matrix effects and limits of quantification (LOQ) were assessed for three matrices within the method validation. The presented method was tested on 41 various feed samples, where presence of PAs was expected.Roughages and feed for rabbits contained the highest levels of PAs, in general.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FOOD CHEMISTRY

ISSN

0308-8146

e-ISSN

1873-7072

Svazek periodika

170

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

265-270

Kód UT WoS článku

000343780400035

EID výsledku v databázi Scopus

2-s2.0-84906965423