UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU112268" target="_blank" >RIV/00216305:26310/14:PU112268 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed

Popis výsledku v původním jazyce

Ergot alkaloids are mycotoxins produced predominantly by fungi of the Claviceps genus, which mainly affect cereals and wild grasses. Ingestion of ergot alkaloids can cause severe health problems in humans and animals. Thus, there is a need for methods that allow determination of these dangerous toxins. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of eight ergot alkaloids: ergosine, ergocornine, ergocryptine and ergocristine andtheir corresponding epimers as well. Mean recovery, precision, matrix effects and limits of quantification (LOQ) have been assessed for cereal and grass matrices within the method validation. Sample preparation has been based on modified QuEChERS approach. The presented method was used to inspect various feed samples. Ergot alkaloids were found in 31 out of 113 samples investigated. Ergocornine was the most frequently occuring alkaloid, while the highest levels were observed for ergocryp

Název v anglickém jazyce

UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed

Popis výsledku anglicky

Ergot alkaloids are mycotoxins produced predominantly by fungi of the Claviceps genus, which mainly affect cereals and wild grasses. Ingestion of ergot alkaloids can cause severe health problems in humans and animals. Thus, there is a need for methods that allow determination of these dangerous toxins. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of eight ergot alkaloids: ergosine, ergocornine, ergocryptine and ergocristine andtheir corresponding epimers as well. Mean recovery, precision, matrix effects and limits of quantification (LOQ) have been assessed for cereal and grass matrices within the method validation. Sample preparation has been based on modified QuEChERS approach. The presented method was used to inspect various feed samples. Ergot alkaloids were found in 31 out of 113 samples investigated. Ergocornine was the most frequently occuring alkaloid, while the highest levels were observed for ergocryp

Druh

D - Stať ve sborníku

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

EMEC15 Book of Abstracts

ISBN

978-80-214-5073-8

ISSN

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e-ISSN

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Počet stran výsledku

523

Strana od-do

113-113

Název nakladatele

VUT Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

3. 12. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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