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Determination of ergot alkaloids in rye flour by ELISA and LC-MS

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F23%3AN0000055" target="_blank" >RIV/60193697:_____/23:N0000055 - isvavai.cz</a>

  • Výsledek na webu

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Determination of ergot alkaloids in rye flour by ELISA and LC-MS

  • Popis výsledku v původním jazyce

    The ergot fungus (Claviceps purupurea) is causative agent of ergot on cereals such as rye, wheat, barley and oats. Spores of this fungus infect cereals and grasses during flowering and produce characteristic black sclerotia (Secale cornutum) which contain varying amounts of ergot alkaloids [1]. From January 1st, 2022, Commission Regulation (EU) No 2021/1399 of 24th August 2021 amending Regulation (EC) No 1881/2006 as regards maximum levels of ergot sclerotia and ergot alkaloids in certain foodstuffs became active. Ground rye products must comply with the limit for ergot alkaloids of 500 μg/kg, which will be further reduced to 250 μg/kg in July 2024. The aim of this study was to determine ergot alkaloids content in commercially available rye flours from Czech Republic by commercially available ELISA kit and by liquid chromatography method coupled with mass spectrometer. A total number of 27 rye flours were tested. The concentration of the sum of alkaloids detected by LC-MS ranged from 0 μg/kg to almost 10000 μg/kg with mean being 783 μg/kg. The ELISA results were generally lower with range from < 29 μg/kg (< LOQ) to 236.9 μg/kg with two samples having concentration higher than detectable limit of 800 μg/kg. Results were presented in the form of a poster at the conference 44. Mycotoxin Workshop, Hannover, Germany, 5-7 June 2023.

  • Název v anglickém jazyce

    Determination of ergot alkaloids in rye flour by ELISA and LC-MS

  • Popis výsledku anglicky

    The ergot fungus (Claviceps purupurea) is causative agent of ergot on cereals such as rye, wheat, barley and oats. Spores of this fungus infect cereals and grasses during flowering and produce characteristic black sclerotia (Secale cornutum) which contain varying amounts of ergot alkaloids [1]. From January 1st, 2022, Commission Regulation (EU) No 2021/1399 of 24th August 2021 amending Regulation (EC) No 1881/2006 as regards maximum levels of ergot sclerotia and ergot alkaloids in certain foodstuffs became active. Ground rye products must comply with the limit for ergot alkaloids of 500 μg/kg, which will be further reduced to 250 μg/kg in July 2024. The aim of this study was to determine ergot alkaloids content in commercially available rye flours from Czech Republic by commercially available ELISA kit and by liquid chromatography method coupled with mass spectrometer. A total number of 27 rye flours were tested. The concentration of the sum of alkaloids detected by LC-MS ranged from 0 μg/kg to almost 10000 μg/kg with mean being 783 μg/kg. The ELISA results were generally lower with range from < 29 μg/kg (< LOQ) to 236.9 μg/kg with two samples having concentration higher than detectable limit of 800 μg/kg. Results were presented in the form of a poster at the conference 44. Mycotoxin Workshop, Hannover, Germany, 5-7 June 2023.

Klasifikace

  • Druh

    O - Ostatní výsledky

  • CEP obor

  • OECD FORD obor

    10406 - Analytical chemistry

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2023

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů