Ergot Alkaloids in Rye Flour Marketed in Czech Republic: Comparison Between ELISA and LC–MS Methodologies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F24%3AN0000007" target="_blank" >RIV/60193697:_____/24:N0000007 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/24:43928942

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12161-024-02612-x" target="_blank" >https://link.springer.com/article/10.1007/s12161-024-02612-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-024-02612-x" target="_blank" >10.1007/s12161-024-02612-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ergot Alkaloids in Rye Flour Marketed in Czech Republic: Comparison Between ELISA and LC–MS Methodologies

Popis výsledku v původním jazyce

Ergot alkaloids (EAs) are toxins produced by Claviceps purpurea fungi that may infect cereals, particularly rye. The present study describes the comparison between ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS) and ELISA for the determination of twelve ergot alkaloids in rye flour. The sample preparation for LC–MS analysis was done by the QuEChERS method and clean-up via freeze-out. The analytical LC–MS method was fully validated, and the validation parameters such as linearity, limit of quantification (LOQ), precision, accuracy and matrix effect were tested. The calibration curves were linear (R2 > 0.99) for all ergot alkaloids. The recoveries ranged from 74 to 104%, and the relative standard deviation under conditions repeatability (RSDr) did not exceed 17% for any analytes. The analytical LC–MS method and ELISA were applied to 27 samples of rye flour commercially available from retail shops in the Czech Republic. A positive correlation was found between the samples measured by ELISA and LC–MS. Ergot alkaloid concentra- tions measured by these methods were compared with the European legislation resulting in 5 samples exceeding the set limit of 500 µg/kg with 2 samples exceeding this limit 10 and 20 times, respectively. With the new legislative limit coming into effect in July 2024, more than half of the samples would not comply with the new established limit of 250 µg/kg.

Název v anglickém jazyce

Ergot Alkaloids in Rye Flour Marketed in Czech Republic: Comparison Between ELISA and LC–MS Methodologies

Popis výsledku anglicky

Ergot alkaloids (EAs) are toxins produced by Claviceps purpurea fungi that may infect cereals, particularly rye. The present study describes the comparison between ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS) and ELISA for the determination of twelve ergot alkaloids in rye flour. The sample preparation for LC–MS analysis was done by the QuEChERS method and clean-up via freeze-out. The analytical LC–MS method was fully validated, and the validation parameters such as linearity, limit of quantification (LOQ), precision, accuracy and matrix effect were tested. The calibration curves were linear (R2 > 0.99) for all ergot alkaloids. The recoveries ranged from 74 to 104%, and the relative standard deviation under conditions repeatability (RSDr) did not exceed 17% for any analytes. The analytical LC–MS method and ELISA were applied to 27 samples of rye flour commercially available from retail shops in the Czech Republic. A positive correlation was found between the samples measured by ELISA and LC–MS. Ergot alkaloid concentra- tions measured by these methods were compared with the European legislation resulting in 5 samples exceeding the set limit of 500 µg/kg with 2 samples exceeding this limit 10 and 20 times, respectively. With the new legislative limit coming into effect in July 2024, more than half of the samples would not comply with the new established limit of 250 µg/kg.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Analytical Methods

ISSN

1936-9751

e-ISSN

1936-976X

Svazek periodika

17

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

787-794

Kód UT WoS článku

001185625000001

EID výsledku v databázi Scopus

2-s2.0-85187948595