Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU119542" target="_blank" >RIV/00216305:26310/15:PU119542 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.fch.vut.cz/media/sok/sbornik_prispevku_2015.pdf" target="_blank" >http://www.fch.vut.cz/media/sok/sbornik_prispevku_2015.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide

Popis výsledku v původním jazyce

This study deals with the effect of hyaluronan addition on the micellar formation of cationic surfactant cetyltrimethylammonium bromide (CTAB) in aqueous solution. Hyaluronan as negatively charged polyelectrolyte can interact with cationic micelles via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. The effect of hyaluronan addition to CTAB solution was study by fluorescence measurement of two fluorescence probes located in different regions of micelles – hydrophobic perylene, which is solubilized in hydrophobic interior and hydrophilic one, fluorescein, which is located on the surface of cationic micelles due to its anionic character. The excitation and emission spectra, intensity of fluorescence, steady-state anisotropy of fluorescence and lifetime of probes in the samples were measured. It is observed, that the addition of hyaluronan to micellar solution of CTAB affected structural changes of microenvironment near the molecules of both fluorescence probes, which are reflected at increasing values of fluorescence anisotropy simultaneously with increasing amount of hyaluronan in solutions.

Název v anglickém jazyce

Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide

Popis výsledku anglicky

This study deals with the effect of hyaluronan addition on the micellar formation of cationic surfactant cetyltrimethylammonium bromide (CTAB) in aqueous solution. Hyaluronan as negatively charged polyelectrolyte can interact with cationic micelles via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. The effect of hyaluronan addition to CTAB solution was study by fluorescence measurement of two fluorescence probes located in different regions of micelles – hydrophobic perylene, which is solubilized in hydrophobic interior and hydrophilic one, fluorescein, which is located on the surface of cationic micelles due to its anionic character. The excitation and emission spectra, intensity of fluorescence, steady-state anisotropy of fluorescence and lifetime of probes in the samples were measured. It is observed, that the addition of hyaluronan to micellar solution of CTAB affected structural changes of microenvironment near the molecules of both fluorescence probes, which are reflected at increasing values of fluorescence anisotropy simultaneously with increasing amount of hyaluronan in solutions.

Druh

D - Stať ve sborníku

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Studentská odborná konference Chemie je život 2015, Sborník příspěvků

ISBN

978-80-214-5290-9

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

244-250

Název nakladatele

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

3. 12. 2015

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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