Hydrophobic nanodomains in hyaluronan-surfactant system: a fluorescence anisotropy study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F16%3APU120727" target="_blank" >RIV/00216305:26310/16:PU120727 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Hydrophobic nanodomains in hyaluronan-surfactant system: a fluorescence anisotropy study

Popis výsledku v původním jazyce

This paper deals with the fluorescence anisotropy study of hydrophobic nanodomains formed by interaction between hyaluronan (Hya) and cationic surfactant cetyltrimethylammonium bromide (CTAB) below critical micelle concentration in aqueous solution and the effect of sodium chloride addition to this system. Hyaluronan as negatively charged polyelectrolyte can interact with cationic surfactants via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. Two fluorescent probes, which are solubilized in hydrophobic region, were used – perylene and diphenylhexatrien (DPH). The emission spectra, total intensity of fluorescence and steady-state anisotropy of fluorescence in the samples were measured. It was observed that initial addition of CTAB to hyaluronan aqueous solution leads to forming some small hydrophobic domains linked to hyaluronan chains. Then an increasing concentration of CTAB causes phase separation and formation of a condensed phase. Addition

Název v anglickém jazyce

Hydrophobic nanodomains in hyaluronan-surfactant system: a fluorescence anisotropy study

Popis výsledku anglicky

This paper deals with the fluorescence anisotropy study of hydrophobic nanodomains formed by interaction between hyaluronan (Hya) and cationic surfactant cetyltrimethylammonium bromide (CTAB) below critical micelle concentration in aqueous solution and the effect of sodium chloride addition to this system. Hyaluronan as negatively charged polyelectrolyte can interact with cationic surfactants via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. Two fluorescent probes, which are solubilized in hydrophobic region, were used – perylene and diphenylhexatrien (DPH). The emission spectra, total intensity of fluorescence and steady-state anisotropy of fluorescence in the samples were measured. It was observed that initial addition of CTAB to hyaluronan aqueous solution leads to forming some small hydrophobic domains linked to hyaluronan chains. Then an increasing concentration of CTAB causes phase separation and formation of a condensed phase. Addition

Druh

O - Ostatní výsledky

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů