Phase Transition of Lipids Membranes Determination: Comparison of Different Methods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F16%3APU119098" target="_blank" >RIV/00216305:26310/16:PU119098 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phase Transition of Lipids Membranes Determination: Comparison of Different Methods

Popis výsledku v původním jazyce

In this work we compare results of phase transition temperature measurements by different methods: microcalorimetry and fluorescence spectroscopy. For this purpose, we prepared the series of lipid mixtures consisting of cationic and zwitterionic lipids with different lipid ratios. Measurements of phase transition temperature by steady-state fluorescence spectroscopy can be utilized prodan and its derivative laurdan. These fluorescence probes are sensitive to the polarity of local environment due to partial charge separation between the 2-dimethylamino and the 6-carbonyl residues forming dipole moment, which causes solvent reorientation upon excitation. This solvent reorientation consumes energy and decreases the probe's excited state energy resulting in continuous red shift of the probe's emission spectrum. Thus, this shift in laurdan and prodan emission spectra indicates the increased concentration of water within the bilayer and its increased mobility.The laurdan in compared to prodan contains lauri

Název v anglickém jazyce

Phase Transition of Lipids Membranes Determination: Comparison of Different Methods

Popis výsledku anglicky

In this work we compare results of phase transition temperature measurements by different methods: microcalorimetry and fluorescence spectroscopy. For this purpose, we prepared the series of lipid mixtures consisting of cationic and zwitterionic lipids with different lipid ratios. Measurements of phase transition temperature by steady-state fluorescence spectroscopy can be utilized prodan and its derivative laurdan. These fluorescence probes are sensitive to the polarity of local environment due to partial charge separation between the 2-dimethylamino and the 6-carbonyl residues forming dipole moment, which causes solvent reorientation upon excitation. This solvent reorientation consumes energy and decreases the probe's excited state energy resulting in continuous red shift of the probe's emission spectrum. Thus, this shift in laurdan and prodan emission spectra indicates the increased concentration of water within the bilayer and its increased mobility.The laurdan in compared to prodan contains lauri

Druh

O - Ostatní výsledky

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů