Polylysine Coated Magnetic Nanoparticles As A Tool For DNA Isolation From Real Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F18%3APU128412" target="_blank" >RIV/00216305:26310/18:PU128412 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polylysine Coated Magnetic Nanoparticles As A Tool For DNA Isolation From Real Samples

Popis výsledku v původním jazyce

Solid phase DNA extraction has been shown to be a simple method for PCR-ready DNA isolation. Poly(Llysine) coated iron oxide nanoparticles (MNPs) were not studied so far. Poly(L-lysine) coated solid surfaces are positively charged in acidic pH and negatively charged in alkali pH due to the protonation and deprotonation of functional groups. This enables the binding of charged species, including nucleic acids, using electrostatic interaction between the solid surface and the charged molecules. First, Nicotiana tabacum DNA capture and elution was studied with different amounts of poly(Llysine) coated magnetic nanoparticles. Buffers with different acidic pH (4.0 – 6.5) and alcalic pH (7.5 - 10.0) were tested for DNA capture or elution, respectively (Figure 1). Next, MNPs were used for isolation of PCRready DNA from plant based foods, in particular from chokeberry, red currant and plum jams which are rich in PCR inhibitors (phenolic compounds and polysacharides) and contain DNA degraded during technologic

Název v anglickém jazyce

Polylysine Coated Magnetic Nanoparticles As A Tool For DNA Isolation From Real Samples

Popis výsledku anglicky

Solid phase DNA extraction has been shown to be a simple method for PCR-ready DNA isolation. Poly(Llysine) coated iron oxide nanoparticles (MNPs) were not studied so far. Poly(L-lysine) coated solid surfaces are positively charged in acidic pH and negatively charged in alkali pH due to the protonation and deprotonation of functional groups. This enables the binding of charged species, including nucleic acids, using electrostatic interaction between the solid surface and the charged molecules. First, Nicotiana tabacum DNA capture and elution was studied with different amounts of poly(Llysine) coated magnetic nanoparticles. Buffers with different acidic pH (4.0 – 6.5) and alcalic pH (7.5 - 10.0) were tested for DNA capture or elution, respectively (Figure 1). Next, MNPs were used for isolation of PCRready DNA from plant based foods, in particular from chokeberry, red currant and plum jams which are rich in PCR inhibitors (phenolic compounds and polysacharides) and contain DNA degraded during technologic

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů