A simple technique for assessing the cuticular diffusion of humic acid biostimulants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU132731" target="_blank" >RIV/00216305:26310/19:PU132731 - isvavai.cz</a>

Výsledek na webu

<a href="https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1" target="_blank" >https://plantmethods.biomedcentral.com/articles/10.1186/s13007-019-0469-x#Abs1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13007-019-0469-x" target="_blank" >10.1186/s13007-019-0469-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A simple technique for assessing the cuticular diffusion of humic acid biostimulants

Popis výsledku v původním jazyce

Background: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing of physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration on humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amounts of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.

Název v anglickém jazyce

A simple technique for assessing the cuticular diffusion of humic acid biostimulants

Popis výsledku anglicky

Background: Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing of physiological effects of such biostimulants which are often supplied as foliar sprays. Therefore, an original experimental method for the qualitative investigation and quantitative description of the penetration on humates through plant cuticles is proposed, tested, and evaluated. Results: The proposed method involves the isolation of model plant leaf cuticles and the subsequent in vitro evaluation of cuticular humate transport. The employed novel methodology is based on a simple diffusion couple arrangement involving continuous spectrophotometric determination of the amounts of penetrated humate in a hydrogel diffusion medium. Prunus laurocerasus leaf cuticles were isolated by chemical and enzymatic treatment and the rate of cuticular penetration of a commercial humate (lignohumate) was estimated over time in quantitative and qualitative terms. Different rates of lignohumate transport were determined for abaxial and adaxial leaf cuticles also in relation to the different cuticular extraction methods tested. Conclusions: The proposed methodology represents a simple and cheap experimental tool for the study on the trans-cuticular penetration of humic-based biostimulants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT METHODS

ISSN

1746-4811

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

1-11

Kód UT WoS článku

000478096200002

EID výsledku v databázi Scopus

2-s2.0-85072153401